{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:48:50Z","timestamp":1790930930057,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,8,5]],"date-time":"2004-08-05T00:00:00Z","timestamp":1091664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Canadian Institutes of Health Research (CIHR)","award":["MOP-36495 (2002-2007)"],"award-info":[{"award-number":["MOP-36495 (2002-2007)"]}]},{"name":"Canadian Institutes of Health Research (CIHR)","award":["MGC-15186 (2001-2006)"],"award-info":[{"award-number":["MGC-15186 (2001-2006)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The unavailability of human cell lines representative of the gastric glandular epithelium while able to form a functional barrier restricts the application of a cell culture approach to the field of gastric epithelial physiology. In the current study, we have characterized new non\u2010transfected clones isolated from gastric carcinoma cell lines known to express functional markers of the human gastric mucosa (<jats:italic>J Cell Biochem<\/jats:italic> 2001;81:241). Twenty\u2010one clones exhibiting epithelial\u2010type junctions (renamed HGE cell lines) were isolated from NCI\u2010N87 (ATCC CRL 5822), whereas only squamous cell lines could be generated from other native strains. Of these 21 clones, HGE\u201017 and HGE\u201020 formed dense coherent monolayers and displayed true epithelial phenotype. E\u2010cadherin and ZO\u20101 proteins were consistently localized at the periphery of all cells which also generated transepithelial electrical resistance. Moreover, growth factors known to be trophic for the gastric mucosa were able to stimulate mitogenesis at subconfluence. HGE\u201017 exhibited a poorly differentiated precursor\u2010like status and responded strongly to EGF\/TGF\u03b1 treatment in restitution assays. HGE\u201020 cells, on the other hand, exhibited a higher degree of differentiation at the ultrastructural level as well as higher gastric lipase and pepsinogen levels. These latter zymogens were compartmentalized into granules which also contained mucin\u20106 (MUC6, prezymogenic\u2010like status). Exogenous hormones, i.e., 1 \u03bcg\/ml hydrocortisone and 5 \u03bcM retinoic acid, significantly increased enzyme levels in HGE\u201020. In conclusion, HGE\u201017 and HGE\u201020 represent the first human gastric cell lines with true epithelial characteristics, opening a venue to important applications for the study of re\u2010epithelization, permeability, and regulation of digestive functions in the context of gastric physiology and pathology. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20124","type":"journal-article","created":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T22:50:17Z","timestamp":1092091817000},"page":"263-274","source":"Crossref","is-referenced-by-count":29,"title":["Establishment of human gastric epithelial (HGE) cell lines exhibiting barrier function, progenitor, and prezymogenic characteristics"],"prefix":"10.1002","volume":"202","author":[{"given":"Pierre","family":"Chailler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"M\u00e9nard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,8,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(91)90165-5"},{"key":"e_1_2_6_3_1","first-page":"1703","article-title":"Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach","volume":"43","author":"Barranco SC","year":"1983","journal-title":"Cancer Res"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(20000301)48:5<293::AID-JEMT6>3.0.CO;2-A"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4711"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4644(20010501)81:2<241::AID-JCB1039>3.0.CO;2-B"},{"key":"e_1_2_6_7_1","first-page":"119","article-title":"Restitution at the cellular level: Regulation of the migratory phenotype","volume":"69","author":"Basson MD","year":"1997","journal-title":"Yale J Biol Med"},{"key":"e_1_2_6_8_1","first-page":"43A","article-title":"[Abstract: Canadian Association of Gastroenterology Meeting] Characterization of the gastric NCI\u2010N87 cell line","volume":"14","author":"Ch\u00e9nard M","year":"2000","journal-title":"Can J Gastroenterol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115189"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015807904558"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","unstructured":"CiacciC LindSE PodolskyDK.1993.Transforming growth factor beta regulation of migration in wounded rat intestinal epithelial monolayers. 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