{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T15:46:08Z","timestamp":1778687168654,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,11,1]],"date-time":"2004-11-01T00:00:00Z","timestamp":1099267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The sensitivity to cholesterol depletion of calcium handling by rat submandibular glands was investigated. The glands were digested with collagenase. After homogenization, the lysate was extracted at 4\u00b0C with 0.5% Triton X\u2010100 and the extract was submitted to an ultracentrifugation in a sucrose discontinuous gradient. A population of detergent\u2010resistant membranes (DRM) was collected at the 5%\u201335% interface. The DRM had a higher content of cholesterol, saturated and long\u2010chain fatty acids. Caveolin\u20101 and \u03b1<jats:sub>q\/11<\/jats:sub> were located in these membranes. They were more ordered than vesicles from total cellular lysate as determined by anisotropy measurement. They disappeared after cholesterol extraction with methyl\u2010\u03b2\u2010cyclodextrin (MCD). Exposure of the cellular suspension with MCD nearly abolished the response to carbachol, epinephrine, and substance P and inhibited the activation of phospholipase C (PLC) by these agonists and by sodium fluoride. MCD did not affect the mobilization of intracellular pools of calcium by thapsigargin. It increased the uptake of extracellular calcium or barium and did not inhibit the uptake of calcium after depletion of the intracellular stores of this ion. From these results, it is concluded that Triton X\u2010100 can extract a fraction of membrane resistant to detergents. Treatment of the cells with MCD disrupts these membranes. The coupling between the heterotrimeric GTP\u2010binding protein G<jats:sub>q\/11<\/jats:sub> and poly\u2010phosphoinositide\u2010specific PLC is affected by disruption of these membrane fractions. At the opposite, the store\u2010operated calcium channel (SOCC) is not affected by DRM\u2010disruption. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20241","type":"journal-article","created":{"date-parts":[[2004,11,2]],"date-time":"2004-11-02T15:48:50Z","timestamp":1099410530000},"page":"429-438","source":"Crossref","is-referenced-by-count":8,"title":["Cholesterol depletion perturbs calcium handling by rat submandibular 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