{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:47:48Z","timestamp":1789674468111,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,4,12]],"date-time":"2005-04-12T00:00:00Z","timestamp":1113264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>JC virus (JCV) causes progressive multifocal leukoencephalopathy (PML), characterized by multiple areas of demyelination and attendant loss of brain function. PML is often associated with immunodepression and it is significantly frequent in AIDS patients. The viral genome is divided into early and late genes, between which lies a non\u2010coding control region (NCCR) that regulates JCV replication and presents a great genetic variability. The NCCR of JCV archetype (CY strain) is divided into six regions: A\u2013F containing binding sites for cell factors involved in viral transcription. Deletions and enhancements of these binding sites characterize JCV variants, which could promote viral gene expression and could be more suitable for the onset or development of PML. Therefore, we evaluated by means of polymerase chain reaction (PCR) the presence of JCV genome in cerebrospinal fluid (CSF) of HIV positive and negative subjects both with PML and after sequencing, we analyzed the viral variants found focusing on Sp1 binding sites (box B and D) and up\u2010TAR sequence (box C). It is known that Sp1 activates JCV early promoter and can contribute in maintaining methylation\u2010free CpG islands in active genes, while up\u2010TAR sequence is important for HIV\u20101 Tat stimulation of JCV late promoter. Our results showed that in HIV\u2010positive subjects all NCCR structures presented enhancements of up\u2010TAR element, whereas in HIV\u2010negative subjects both Sp1 binding sites were always retained. Therefore, we can support the synergism HIV\u20101\/JCV in CNS and we can hypothesize that both Sp1 binding sites could be important to complete JCV replication cycle in absence of HIV\u2010coinfection. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20375","type":"journal-article","created":{"date-parts":[[2005,4,12]],"date-time":"2005-04-12T16:49:41Z","timestamp":1113324581000},"page":"913-918","source":"Crossref","is-referenced-by-count":23,"title":["Investigation on the role of cell transcriptional factor Sp1 and HIV\u20101 TAT protein in PML onset or 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