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In this study, intrahepatic and I.V. injections of a double\u2010stranded oligodeoxynucleotide decoy containing the TGF\u2010\u03b2 regulatory element found in the distal promoter of the <jats:italic>COL1A1<\/jats:italic> gene into worm\u2010infected mice suppressed TGF\u2010\u03b21, COL1A1, tissue inhibitor of metalloproteinase\u20101, and decreased COL3A1 mRNAs to a lesser extent. Sequence comparisons within the mouse genome found homologous sequences within the COL3A1, TGF\u2010\u03b21, and TIMP\u20101 5\u2032 flanking regions. Cold competition gel mobility shift assays using these homologous sequences with 5\u2032 and 3\u2032 flanking regions found in the natural <jats:italic>COL1A1<\/jats:italic> gene showed competition. Competitive gel mobility assays in a separate experiment showed no competition using a 5\u2010base mutated or scrambled sequence. Explanted liver granulomas from saline\u2010injected mice incorporated 10.45\u2009\u00b1\u20091.7% <jats:sup>3<\/jats:sup>H\u2010proline into newly synthesized collagen, whereas decoy\u2010treated mice showed no collagen synthesis. Compared with the saline control schistosomiasis mice phosphorothioate double\u2010stranded oligodeoxynucleotide treatment decreased total liver collagen content (i.e. hydroxy\u20104\u2010proline) by 34%. This novel molecular approach has the potential to be employed as a novel antifibrotic treatment modality. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20412","type":"journal-article","created":{"date-parts":[[2005,5,7]],"date-time":"2005-05-07T00:48:01Z","timestamp":1115426881000},"page":"370-374","source":"Crossref","is-referenced-by-count":7,"title":["A novel nonsteroidal antifibrotic oligo decoy containing the TGF\u2010\u03b2 element found in the <i>COL1A1<\/i> gene which regulates murine schistosomiasis liver 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