{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:55:19Z","timestamp":1761216919987},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T00:00:00Z","timestamp":1126483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nitric oxide (NO) is a chemical messenger implicated in neuronal damage associated with ischemia neurodegenerative disease and excitotoxicity. In the present study, we examined the biological effects of NO and its mechanisms in human malignant glioblastoma cells. Addition of a NO donor, <jats:italic>S<\/jats:italic>\u2010nitroso\u2010<jats:italic>N<\/jats:italic>\u2010acetyl\u2010penicillamine (SNAP), induced apoptosis in U87MG human glioblastoma cells, accompanied by opening mitochondrial permeability transition pores, release of cytochrome c and AIF, and subsequently by caspase activation. NO\u2010induced apoptosis occurred concurrently with significantly increased levels of the Bak and Bim. Treatment with SNAP resulted in sustained activation of JNK and its downstream pathway, c\u2010Jun\/AP\u20101. The expression of dominant\u2010negative (DN)\u2010JNK1 and DN\u2010c\u2010Jun suppressed the activation of AP\u20101, the induction of Bak and Bim, and the SNAP\u2010induced apoptosis. In addition, de novo protein synthesis was required for the initiation of apoptosis in that the protein synthesis inhibitor, cycloheximide (CHX), inhibited NO\u2010induced apoptotic cell death as well as up\u2010regulation of Bak and Bim. These results suggest that NO activates an apoptotic cascade, involving sustained JNK activation, AP\u20101 DNA binding activity, and subsequent Bak and Bim induction, followed by cytochrome c and AIF releases and caspases cascade activation, resulting in human malignant brain tumor cell death. J. Cell. Physiol. 206: 477\u2013486, 2006. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20488","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T21:48:27Z","timestamp":1126561707000},"page":"477-486","source":"Crossref","is-referenced-by-count":47,"title":["Up\u2010regulation of Bak and Bim via JNK downstream pathway in the response to nitric oxide in human glioblastoma cells"],"prefix":"10.1002","volume":"206","author":[{"given":"Hyeon\u2010Ok","family":"Jin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In\u2010Chul","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungkwan","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung\u2010Chahn","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang\u2010Hyeok","family":"Woo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010Joon","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Jae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung\u2010Jin","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doo\u2010Hyun","family":"Yoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Hun","family":"Rhee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok\u2010Il","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,9,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90143-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/6854"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.8.1567"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401400"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00320-3"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc883"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7901908"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231177098"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-0618(96)00148-2"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00728-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2003.08.011"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.05.048"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7824938"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-14-05124.1998"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.1.21"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02319.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.24.2996"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1830"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2003.08.009"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/74994"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.1997.0118"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80727-8"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2003.11.009"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400580"},{"key":"e_1_2_6_26_1","first-page":"7310","article-title":"Bak: A downstream mediator of fenretinide\u2010induced apoptosis of SH\u2010SY5Y neuroblastoma cells","volume":"63","author":"Lovat PE","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00845-6"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07193.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019052-199512000-00016"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/02688690120057628"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0844(199809)16:3<183::AID-CBF784>3.0.CO;2-0"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-02-00751.1998"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059108"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00239-2"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20433"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/39899"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.897601"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.20488","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.20488","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:33:47Z","timestamp":1695947627000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.20488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9,12]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1002\/jcp.20488"],"URL":"https:\/\/doi.org\/10.1002\/jcp.20488","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,9,12]]}}}