{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T06:39:27Z","timestamp":1778395167259,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T00:00:00Z","timestamp":1129852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have identified several cytokines as inducers of tenascin\u2010C (TN\u2010C) expression in various tissue culture systems. However, the signaling pathways of the regulation of TN\u2010C expression are almost unknown. In this study, we clarified the molecular mechanism(s) underlying the regulation of the <jats:italic>TN\u2010C<\/jats:italic> gene by platelet derived growth factor (PDGF) in cultured human dermal fibroblasts. PDGF induced the expression of TN\u2010C protein as well as mRNA in a dose\u2010dependent manner. Actinomycin D, an RNA synthesis inhibitor, significantly blocked the PDGF\u2010mediated upregulation of TN\u2010C mRNA expression, whereas cycloheximide, a protein synthesis inhibitor, did not. The PDGF\u2010mediated induction of TN\u2010C expression was inhibited by the treatment of fibroblasts with a selective phosphoinositide 3\u2010kinase (PI3K) inhibitor, wortmannin, or LY294002. These results suggest that PDGF induced the expression of TN\u2010C at a transcriptional level via phosphoinositide3\u2010kinase\/Akt signaling pathways. We performed serial 5\u2032 deletions and a transient transfection analysis to define the region in the TN\u2010C promoter mediating the responsiveness to PDGF. Overexpression of Sp1, Ets1, or Ets2 activated the TN\u2010C promoter and superinduced TN\u2010C promoter activity stimulated by PDGF, whereas overexpression of Fli1 inhibited the effects of PDGF on TN\u2010C expression. Mutation of the Sp1\/3 binding sites or Ets binding sites in the TN\u2010C promoter region responsible to PDGF abrogated the PDGF\u2010inducible promoter activity. Immunoprecipitation analysis revealed that Sp1, Ets1, and Ets2 form a transcriptionally active complex. On the other hand, the interaction of Fli1 with Sp1 decreased after PDGF treatment. These results suggest that the upregulation of TN\u2010C expression by PDGF involves Ets family transcription factors, co\u2010operating with Sp1. J.Cell.Physiol. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20527","type":"journal-article","created":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T20:19:33Z","timestamp":1129925973000},"page":"718-727","source":"Crossref","is-referenced-by-count":37,"title":["Platelet derived growth factor induced tenascin\u2010C transcription is phosphoinositide 3\u2010kinase\/Akt\u2010dependent and mediated by Ets family transcription factors"],"prefix":"10.1002","volume":"206","author":[{"given":"Masatoshi","family":"Jinnin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Ihn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihide","family":"Asano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Yamane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Trojanowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunihiko","family":"Tamaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01045.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.50.31515"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.5.3230"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/321702a0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/15419069409097262"},{"key":"e_1_2_6_7_1","first-page":"942","article-title":"A simplification of the nuclear run\u2010off transcription assay","volume":"7","author":"Celano P","year":"1989","journal-title":"BioTechniques"},{"key":"e_1_2_6_8_1","first-page":"67","article-title":"Tenascin\u2010C in peripheral nerve morphogenesis","volume":"2","author":"Chiquet M","year":"1994","journal-title":"Dev Neurobiol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.08.001"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2001.117721"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19960616)61:4<592::AID-JCB13>3.0.CO;2-I"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010133200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200206200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH752>3.0.CO;2-V"},{"key":"e_1_2_6_15_1","first-page":"71","article-title":"Stimulation of tenascin expression in mesenchyme by epithelial\u2010mesenchymal interactions","volume":"33","author":"Ekblom P","year":"1989","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(93)90037-Q"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh2301.099642"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.7.3429"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.5.1588"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06695.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2836952"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.1.8.555"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/319511a0"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02955.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-419X(98)00015-8"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.46.29342"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00956.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.43.26717"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24666"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.4.1895"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207064"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(01)00103-4"},{"key":"e_1_2_6_33_1","first-page":"56","article-title":"Stimulation of repair in chronic, nonhealing, cutaneous ulcers using platelet\u2010derived wound healing formula","volume":"170","author":"Knighton DR","year":"1990","journal-title":"Surg Gynecol Obstet"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00401.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(97)00031-9"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12331160"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780351011"},{"key":"e_1_2_6_38_1","first-page":"1024","article-title":"Nuclear runoff transcription analysis using chemiluminescent detection","volume":"16","author":"Merscher S","year":"1994","journal-title":"Biotechniques"},{"key":"e_1_2_6_39_1","first-page":"439","article-title":"Appearance of tenascin in healing skin of the mouse: Possible involvement in seaming of wounded tissues","volume":"33","author":"Murakami R","year":"1989","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910470603"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.12.2685108"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)81860-9"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202220"},{"key":"e_1_2_6_44_1","doi-asserted-by":"crossref","first-page":"23910","DOI":"10.1016\/S0021-9258(18)35923-4","article-title":"Angiotensin II regulates tenascin gene expression in vascular smooth muscle cells","volume":"267","author":"Sharifi BG","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203360"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909023411006"},{"key":"e_1_2_6_47_1","doi-asserted-by":"crossref","first-page":"2963","DOI":"10.1002\/j.1460-2075.1984.tb02241.x","article-title":"Purification and properties of porcine platelet\u2010derived growth factor","volume":"3","author":"Stroopbant P","year":"1984","journal-title":"EMBO J"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.104.1.69"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080390214"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104170200"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11029"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.20527","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.20527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T20:29:22Z","timestamp":1696796962000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.20527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10,21]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1002\/jcp.20527"],"URL":"https:\/\/doi.org\/10.1002\/jcp.20527","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10,21]]}}}