{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T22:58:48Z","timestamp":1779231528607,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,10,31]],"date-time":"2005-10-31T00:00:00Z","timestamp":1130716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human lymphatic endothelial cells (LECs) have isolated prevalently from human derma and tumors. As specialized lymphatic organs within the oropharynx, palatine tonsils are easily obtained and rich in lymphatic venules. Using a two\u2010step purification method based on the sorting of endothelial cells with Ulex Europaeus Agglutinin 1 (UEA\u20101)\u2010coated beads, followed by purification with monoclonal antibody D2\u201340, we successfully purified LECs from human palatine tonsils. The LECs were expanded on flasks coated with collagen type 1 and fibronectin for up to 8\u201310 passages and then analyzed for phenotypic and functional properties. Cultured cells retained the phenotypic pattern of the lymphatic endothelium of palatine tonsils and expressed functional VEGFR\u20103 molecules. In fact, stimulation with VEGFR\u20103 ligand, the vascular endothelium grow factor C, induced a marked increase in cell proliferation. Similarly to blood endothelial cells (BECs), LECs were able to form tube\u2010like structure when seeded in Cultrex basement membrane extract. Comparative studies performed on LECs derived from palatine tonsils and iliac lymphatic vessels (ILVs), obtained with the same procedures, showed substantial discrepancies in the expression of various lymphatic markers. This points to the existence of micro\u2010 and macrovessel\u2010derived LECs with different phenotypes, possibly involving different biological activities and functions. Palatine tonsil\u2010 and ILV\u2010derived LECs may, therefore, represent new models for investigating function and biochemical properties of these lymphatic endothelia. J. Cell. Physiol. 207: 107\u2013113, 2006. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20537","type":"journal-article","created":{"date-parts":[[2005,10,31]],"date-time":"2005-10-31T21:46:35Z","timestamp":1130795195000},"page":"107-113","source":"Crossref","is-referenced-by-count":31,"title":["Isolation and characterization of lymphatic microvascular endothelial cells from human 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