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The present investigation started from the observation that L\u2010ferritin down\u2010regulated LM cells displayed a less pigmented phenotype, confirmed by a major decrease of total melanin, when compared to control LM cells. This finding was accompanied by a dramatic decrease in tyrosinase activity, which was not paralleled by a concomitant reduction of the amount of tyrosinase specific mRNA. Western blot analysis of tyrosinase in control LM cells displayed a pattern, which corresponds to the progressive glycosylation of the native protein up to the 80 kDa form, considered the functional one. Tyrosinase pattern assayed in L\u2010ferritin down\u2010regulated LM cells showed the remarkable absence of the 80 kDa form and a prevalence of endoglycosidase H (endo H)\u2010sensitive immature (70 kDa) tyrosinase, accumulated in the endoplasmic reticulum (ER), as confirmed by confocal microscopy analysis. These results demonstrate that, in a human metastatic melanoma cell line, the stress condition promoted by L\u2010ferritin down\u2010modulation, can substantially influence proper maturation of tyrosinase. J. Cell. Physiol. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20543","type":"journal-article","created":{"date-parts":[[2005,10,26]],"date-time":"2005-10-26T16:16:48Z","timestamp":1130343408000},"page":"843-848","source":"Crossref","is-referenced-by-count":23,"title":["Ferritin light chain down\u2010modulation generates depigmentation in human metastatic melanoma cells by influencing tyrosinase maturation"],"prefix":"10.1002","volume":"206","author":[{"given":"Vittoria","family":"Maresca","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrica","family":"Flori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giorgia","family":"Cardinali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Briganti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Lombardi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Maria","family":"Mileo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco G.","family":"Paggi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Picardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1434"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)33493-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2003.00026.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0631"},{"key":"e_1_2_6_6_1","first-page":"4041","article-title":"Comparison of the responses of human melanocytes with different melanin contents to ultraviolet B irradiation","volume":"55","author":"Barker D","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2000.130203.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2002.02004.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.08.010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2003.11.005"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2002.02012.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6210"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.11.5889"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008703200"},{"key":"e_1_2_6_16_1","volume-title":"Antibodies: A laboratory manual","author":"Harlow E","year":"1988"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.03952.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.12.2335"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00126-0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-419X(02)00068-9"},{"key":"e_1_2_6_21_1","first-page":"2588","article-title":"Increase of cisplatin sensitivity by c\u2010myc antisense oligodeoxynucleotides in a human metastatic melanoma inherently resistant to cisplatin","volume":"5","author":"Leonetti C","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.4.7.679"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(98)00092-6"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12335801"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2550139.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-3084(03)00089-6"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140303.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(01)00650-5"},{"key":"e_1_2_6_29_1","first-page":"219","article-title":"Mechanisms for post\u2010inflammatory hyperpigmentation and hypopigmentation","volume":"256","author":"Nordlund JJ","year":"1988","journal-title":"Prog Clin Biol Res"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300658200"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"12586","DOI":"10.1016\/S0021-9258(18)90787-8","article-title":"Glycosylation of the epidermal growth factor receptor in A\u2010431 cells. 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