{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T02:02:16Z","timestamp":1770429736765,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,5]],"date-time":"2005-12-05T00:00:00Z","timestamp":1133740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There are two known phosphorylation\u2010mediated inactivation mechanisms for TRPC3 channels. Protein kinase G (PKG) inactivates TRPC3 by direct phosphorylation on Thr\u201011 and Ser\u2010263 of the TRPC3 proteins, and protein kinase C (PKC) inactivates TRPC3 by phosphorylation on Ser\u2010712. In the present study, we explored the relationship between these two inactivation mechanisms of TRPC3. HEK cells were first stably transfected with a PKG\u2010expressing construct and then transiently transfected with a TRPC3\u2010expressing construct. Addition of 1\u2010oleoyl\u20102\u2010acetyl\u2010<jats:italic>sn<\/jats:italic>\u2010glycerol (OAG), a membrane\u2010permeant analog of diacylglycerol (DAG), elicited a TRPC3\u2010mediated [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> rise in these cells. This OAG\u2010induced rise in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> could be inhibited by phorbol 12\u2010myristate 13\u2010acetate (PMA), an agonist for PKC, in a dose\u2010dependent manner. Importantly, point mutations at two PKG phosphorylation sites (T11A\u2010S263Q) of TRPC3 markedly reduced the PMA inhibition. Furthermore, inhibition of PKG activity by KT5823 (1 \u00b5M) or H8 (10 \u00b5M) greatly reduced the PMA inhibition of TRPC3. These data strongly suggest that the inhibitory action of PKC on TRPC3 is partly mediated through PKG in these PKG\u2010overexpressing cells. The importance of this scheme was also tested in vascular endothelial cells, in which PKG plays a pivotal functional role. In these cells, OAG\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> rise was inhibited by PMA, which activates PKC, and by 8\u2010BrcGMP and S\u2010nitroso\u2010<jats:italic>N<\/jats:italic>\u2010acetylpenicillamine (SNAP), both of which activate PKG. Importantly, the PMA inhibition on OAG\u2010induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> rise was significantly reduced by PKG inhibitor KT5823 (1 \u00b5M) or DT\u20103 (500 nM), suggesting an important role of PKG in the PMA\u2010induced inhibition of TRPC channels in native endothelial cells. J. Cell. Physiol. 207: 315\u2013321, 2006. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20567","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:47:41Z","timestamp":1133830061000},"page":"315-321","source":"Crossref","is-referenced-by-count":42,"title":["Protein kinase C can inhibit TRPC3 channels indirectly via stimulating protein kinase G"],"prefix":"10.1002","volume":"207","author":[{"given":"Hiu\u2010Yee","family":"Kwan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqiang","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313975200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14772"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.5.897"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00338.2003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000100390.68771.CC"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0723"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311274200"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/16711"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300045200"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304487200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/24890"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.10.6758"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0304471101"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(03)00060-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5458.1647"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00001.2002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/stke.2722005re3"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.07.037"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10623320303356"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411089200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/0470862580.ch9"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.32.20029"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300544200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.007252"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302162200"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405280200"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302751200"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0303329101"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110881200"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211061200"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(03)00122-6"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0720-y"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008914200"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051632698"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.20567","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.20567","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:42:33Z","timestamp":1696898553000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.20567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,5]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1002\/jcp.20567"],"URL":"https:\/\/doi.org\/10.1002\/jcp.20567","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12,5]]}}}