{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T10:08:26Z","timestamp":1791108506455,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T00:00:00Z","timestamp":1164672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Increased myometrial expression of cyclooxygenase\u20102 (Cox\u20102) at term results from elevated local levels of inflammatory cytokines, and its inhibition provides a potential route for intervention in human pre\u2010term labor. We have identified a role for atypical protein kinase C (PKC) isozymes in IL\u20101\u03b2\u2010induced Cox\u20102 expression in human myometrial smooth muscle cells (HMSMC). The PKC inhibitor GF109203X (10 \u00b5M) inhibited IL\u20101\u03b2\u2010induced Cox\u20102 protein and RNA expression, which were also reduced by MAPK and nuclear factor \u03baB (NF\u2010\u03baB) inhibitors. GF109203X did not affect MAPK activities, and neither did it replicate the effect of p38 MAPK inhibition on Cox\u20102 mRNA stability, suggesting that PKC operates through an independent mechanism. The effect of GF109203X remained intact after depletion of conventional and novel PKC isozymes by phorbol ester pre\u2010treatment. In contrast LY379196 (10 \u00b5M), which at micromolar concentrations inhibits all but atypical PKCs, did not affect Cox\u20102 expression. A peptide corresponding to the pseudosubstrate sequence of atypical PKCs blocked Cox\u20102 protein expression, whereas the sequence from conventional PKCs was ineffective. GF109203X did not affect NF\u2010\u03baB binding to nuclear proteins, but strongly reduced NF\u2010\u03baB\u2010dependent transcription in luciferase reporter assays. Our findings indicate that IL\u20101\u03b2\u2010induced Cox\u20102 expression in HMSMC in culture requires p38\u2010MAPK\u2010mediated mRNA stabilization and an independent activation of Cox\u20102 transcription which is dependent on the action of atypical PKCs, probably through direct stimulation of the transactivating activity of NF\u2010\u03baB. J. Cell. Physiol. 210: 637\u2013643, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20901","type":"journal-article","created":{"date-parts":[[2006,11,28]],"date-time":"2006-11-28T18:57:40Z","timestamp":1164740260000},"page":"637-643","source":"Crossref","is-referenced-by-count":25,"title":["Role of atypical protein kinase C isozymes and NF\u2010\u03baB in IL\u20101\u03b2\u2010induced expression of cyclooxygenase\u20102 in human myometrial smooth muscle cells"],"prefix":"10.1002","volume":"210","author":[{"given":"Sara V.","family":"Duggan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamsin","family":"Lindstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Iglesias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip 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