{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:13:05Z","timestamp":1760548385410},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T00:00:00Z","timestamp":1165968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stem cell homing has been studied in syngeneic models and appears to be rapid (&lt;1 h) and dependent on cellular adhesion and migration factors. We utilized a full H2\u2010mismatched transplantation model to determine the basics of allogeneic homing. C57BL\/6J Lin\u2010Sca\u20101+ cells were labeled with CFSE and injected in non\u2010myeloablated BALB\/c mice. Fluorescent cell detection was via high\u2010speed FACS analysis. Alternatively, B6.SJL whole bone marrow cells were injected in lethally irradiated BALB\/c mice (10 Gy). One, 3, 6, and 24 h after transplant, marrow was harvested and cells were either plated for high proliferative potential colony\u2010forming cell (HPP\u2010CFC) assay or secondarily injected into myeloablated (8 Gy) C57BL\/6J mice using 10% competing C57BL\/6J marrow. Chimerism was evaluated at 8 weeks. CFSE+ cells were detected in the bone marrow 1, 3, and 6 h after injection. The numbers were moderately lower when compared to syngeneic homing possibly due to strain effect. Conversely, utilizing a surrogate or secondary assay, we observed a decline of secondary engraftment of harvested cells over time, but not of HPP\u2010CFC. Combining experiments and normalizing the 1\u2010h time point to 100% (to allow comparison), we observed a mean relative engraftment of 87\u2009\u00b1\u200929%, 72\u2009\u00b1\u200921%, 84\u2009\u00b1\u200935% of the 1 h level at 3, 6, and 24 h respectively. HPP\u2010CFC assay showed no significant variation as a homing surrogate over 1\u20136 h. These data indicate a rapid homing into allogeneic recipients with a plateau at 1 h. The decline of secondary engraftability over time may indicate a phenotype alteration of homed cells. J. Cell. Physiol. 211: 386\u2013391, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.20945","type":"journal-article","created":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T17:50:17Z","timestamp":1166032217000},"page":"386-391","source":"Crossref","is-referenced-by-count":19,"title":["Murine allogeneic in vivo stem cell homing<sup>,<\/sup>"],"prefix":"10.1002","volume":"211","author":[{"given":"Gerald A.","family":"Colvin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Francois","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark S.","family":"Dooner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Cerny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Quesenberry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04247"},{"key":"e_1_2_5_3_1","first-page":"1","volume-title":"Statistical Methods in Medical Research","author":"Armitage P","year":"2001"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04114.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1083-8791(03)00150-2"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-10-3611"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(98)00037-X"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-76912-2_7"},{"key":"e_1_2_5_9_1","first-page":"999","article-title":"Multiparameter analysis of transplantable hemopoietic stem cells: I. 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