{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:00:16Z","timestamp":1789992016130,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,4,18]],"date-time":"2007-04-18T00:00:00Z","timestamp":1176854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When examining HEK293 cells by whole\u2010cell patch\u2010clamp electrophysiology we found spontaneous currents, present in almost all cells. These currents were carried by Na<jats:sup>+<\/jats:sup> ions, were inhibited by amiloride and by cells exposure to acidic (pH 6.3) extracellular solutions. These properties (ion carrier, amiloride\u2010sensitivity, and inactivation by constant lowering of extracellular pH) were similar to the properties of proton\u2010activated currents measured from the same cells. Spontaneous currents required intracellular ATP, were completely inhibited by intracellular Ca<jats:sup>2+<\/jats:sup> buffering with BAPTA and were suppressed by intracellular administration of vesicular H<jats:sup>+<\/jats:sup>ATPase inhibitor bafilomycin. ATP\u2010induced Ca<jats:sup>2+<\/jats:sup> influx through P2X receptors in HEK293 cells stably transfected with P2X<jats:sub>2<\/jats:sub>, P2X<jats:sub>2\/3<\/jats:sub> or P2X<jats:sub>4<\/jats:sub> purinoreceptor subunits transiently potentiated amplitude and frequency of spontaneous currents; this effect was antagonized by bafilomycin. We concluded that spontaneous currents represent activation of acid\u2010sensitive ion channels (ASICs) by autocrine vesicular release of protons from HEK cells. \u00a9 2007 Wiley\u2010Liss, Inc. J. Cell. Physiol. 212: 473\u2013480, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21043","type":"journal-article","created":{"date-parts":[[2007,4,18]],"date-time":"2007-04-18T19:51:31Z","timestamp":1176925891000},"page":"473-480","source":"Crossref","is-referenced-by-count":10,"title":["Spontaneous autocrine release of protons activates ASIC\u2010mediated currents in HEK293 cells"],"prefix":"10.1002","volume":"212","author":[{"given":"Ulyana","family":"Lalo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Pankratov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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