{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:21:30Z","timestamp":1779175290942,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,4,18]],"date-time":"2007-04-18T00:00:00Z","timestamp":1176854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The protein bullous pemphigoid antigen\u20102 (BPAG2\/BP180\/collagen type XVII) plays a key role in attachment of basal keratinocytes to epidermal basement membrane. The binding of BP180 with either integrin \u03b16, integrin \u03b24, or bullous pemphigoid antigen\u20101 (BPAG1\/BP230) is critical for this attachment in skin. The protein 14\u20103\u20103 \u03c3, also known as stratifin and a marker for epithelial cells, is a member of a highly conserved small acidic 14\u20103\u20103 protein family naturally found in all eukaryotic cells. Here, we have used a 14\u20103\u20103\u03c3 GST pull\u2010down screening assay and showed that sigma (\u03c3) isoform of the 14\u20103\u20103 protein family interacts with the cytoplasmic N\u2010terminal domain of BP180. Analysis of a series of truncated or deleted 14\u20103\u20103\u03c3 revealed that only intact 14\u20103\u20103\u03c3 molecule, but not any of its fragments can interact with BP180. This finding suggests that conformation and possible dimerization of 14\u20103\u20103 \u03c3 is essential for this interaction. Further, a BP180 co\u2010immunoprecipitation (IP) and its reverse IP assays were conducted and the results confirmed that 14\u20103\u20103 \u03c3 interacts with cytoplasmic domain, but not ecto\u2010domain of the BP180. In conclusion, the finding of this study provides evidence that 14\u20103\u20103\u03c3 isoform interacts with BP180 which is a major component of hemidesmosome involved in the attachment of epidermis to the basement membrane in skin. However, the significance of this interaction in hemidesmosome formation and\/or attachment needs to be explored. J. Cell. Physiol. 212:675\u2013681, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21064","type":"journal-article","created":{"date-parts":[[2007,4,18]],"date-time":"2007-04-18T19:49:44Z","timestamp":1176925784000},"page":"675-681","source":"Crossref","is-referenced-by-count":9,"title":["14\u20103\u20103 sigma isoform interacts with the cytoplasmic domain of the transmembrane BP180 in keratinocytes"],"prefix":"10.1002","volume":"212","author":[{"given":"Yunyuan","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruhangiz T.","family":"Kilani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan C.R.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aziz","family":"Ghahary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19990301)72:3<356::AID-JCB5>3.0.CO;2-M"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12329728"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.6.1333"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00165.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.2869"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1995.tb02665.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","unstructured":"FuH SubramanianRR MastersSC.2000.14\u20103\u20103 proteins: structure function and regulation.Annu Rev Pharmacol Toxicol40:617\u2013647.","DOI":"10.1146\/annurev.pharmtox.40.1.617"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22519.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12616580"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19981101)43:3<207::AID-JEMT2>3.0.CO;2-Z"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.9711"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.1.277"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12616615"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00572.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(92)90034-6"},{"key":"e_1_2_6_17_1","first-page":"70","article-title":"A hemidesmosomal transmembrane collagenous molecule, the 180\u2010kDa bullous pemphigoid antigen (BPA II), is phosphorylated with 12\u2010O\u2010tetradecanoylphorbol\u201013\u2010acetate in a human squamous cell carcinoma cell line (DJM\u20101)","volume":"4","author":"Kitajima Y","year":"1995","journal-title":"Epithelial Cell Biol"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00241"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23765.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"8825","DOI":"10.1016\/S0021-9258(18)52948-3","article-title":"Cloning of type XVII collagen. Complementary and genomic DNA sequences of mouse 180\u2010kilodalton bullous pemphigoid antigen (BPAG2) predict an interrupted collagenous domain, a transmembrane segment, and unusual features in the 5\u2032\u2010end of the gene and the 3\u2032\u2010untranslated region of the mRNA","volume":"268","author":"Li K","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.2.345"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116856"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/376191a0"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12345821"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81067-3"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410000197"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00201-6"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.40.25937"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00141.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02401.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I"},{"key":"e_1_2_6_32_1","first-page":"37","article-title":"BP180 (type XVII collagen) and its role in cutaneous biology and disease","volume":"19","author":"Van den Bergh F","year":"2003","journal-title":"Adv Dermatol"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3450297"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.33.20029"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/376188a0"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80487-0"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(99)00019-5"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.21064","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.21064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T21:09:09Z","timestamp":1697144949000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.21064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,18]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/jcp.21064"],"URL":"https:\/\/doi.org\/10.1002\/jcp.21064","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,18]]}}}