{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:35:31Z","timestamp":1762144531373},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,5,24]],"date-time":"2007-05-24T00:00:00Z","timestamp":1179964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Smad1 is a receptor\u2010activated intracellular signaling protein, which mediates signal transduction of bone morphogenetic proteins. Current study investigated the expression and transcriptional activity of Smad1 during hepatic stellate cell (HSC) activation. Rat HSCs were isolated from rats at 1, 2, 3 and 4 days after gavaged with carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) or corn oil. RT\u2010PCR, Western blot, gel\u2010shift assay and luciferase assay were employed to examine Smad1 expression and transcriptional activity, respectively. CCl<jats:sub>4<\/jats:sub>\u2010cirrhotic liver fat\u2010storing cells\u20108B (CFSC\u20108B) cells were infected with recombinant adenoviruses of Smad1 and\/or Smad1 shRNA. Both mRNA and protein levels of Smad1 were significantly increased at 48 h after gavage of CCl<jats:sub>4<\/jats:sub>. Gel shift assays demonstrated a significant increase in nuclear Smad1 in day 9 HSCs. Transfection of HSCs with Smad1 responsible luciferase indicated an increase in Smad1 transcriptional activity in day 6 HSCs (1.563\u2009\u00b1\u20090.229 in day 6 versus 0.785\u2009\u00b1\u20090.192 in day 3). When CFSC\u20108B cells were infected with adenoviruses with Smad1 or Smad1 short hairpin RNA (shRNA), there was an increase or decrease in Smad1 mRNA and protein, respectively. Smooth muscle \u03b1\u2010actin expression was increased or decreased according to induction or reduction of Smad1. In conclusion, there were significantly increases in Smad1 expression and transcriptional activity during in vivo activation of hepatic stellate cells. J. Cell. Physiol. 212:764\u2013770, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21074","type":"journal-article","created":{"date-parts":[[2007,5,27]],"date-time":"2007-05-27T05:05:37Z","timestamp":1180242337000},"page":"764-770","source":"Crossref","is-referenced-by-count":12,"title":["Increased Smad1 expression and transcriptional activity enhances trans\u2010differentiation of hepatic stellate cells"],"prefix":"10.1002","volume":"212","author":[{"given":"Hong","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianghong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Burczynski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald 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