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In this report, we showed that arylsulfatase A (AS\u2010A), existing on the sperm head surface, also had this dispersion action. Purified AS\u2010A free of protease, hyaluronidase and chondroitinase activities could disperse the cumulus matrix of expanded COCs. However, this COC dispersion action was not associated with AS\u2010A desulfation activity, assayed by using <jats:italic>p<\/jats:italic>\u2010nitrocatecholsulfate (artificial substrate). COCs incubated for 1 h with sperm pretreated with anti\u2010AS\u2010A IgG in the presence of apigenin (a hyaluronidase inhibitor) did not exhibit matrix dispersion, whereas several cumulus layers were already dispersed in COCs incubated with sperm pretreated with preimmune IgG. Furthermore, sperm from <jats:italic>AS\u2010A<\/jats:italic> null mice showed a significant delay in COC dispersion, compared with wild\u2010type sperm. Within 1 h of sperm\u2010COC co\u2010incubation, the size of COCs incubated with <jats:italic>AS\u2010A<\/jats:italic> null sperm was 65% of the original dimension, whereas that of COCs inseminated with wild\u2010type sperm was only 17%. A further delay in COC dispersion by <jats:italic>AS\u2010A<\/jats:italic>(\u2212\/\u2212) mouse sperm was observed when apigenin was present in the co\u2010incubation. We also showed for the first time that AS\u2010A had a specific affinity for chondroitin sulfate B, a component of cumulus matrix proteoglycan networks; this might provide a mechanism of cumulus matrix destabilization induced by sperm surface AS\u2010A. J. Cell. Physiol. 213: 201\u2013211, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21113","type":"journal-article","created":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T16:18:26Z","timestamp":1178036306000},"page":"201-211","source":"Crossref","is-referenced-by-count":24,"title":["Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes"],"prefix":"10.1002","volume":"213","author":[{"given":"Alexander","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Araya","family":"Anupriwan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sitthichai","family":"Iamsaard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krittalak","family":"Chakrabandhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela 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