{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T20:52:35Z","timestamp":1784235155318,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,7,17]],"date-time":"2008-07-17T00:00:00Z","timestamp":1216252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Runx2 is a master transcription factor for chondrocyte and osteoblast differentiation and bone formation. However expression of Runx2 (by RT\u2010PCR), has been reported in non\u2010skeletal tissues such as breast, T cells and testis. To better define Runx2 activity in non\u2010skeletal tissues, we examined transgenic (Tg) mice expressing <jats:italic>LacZ<\/jats:italic> gene under control of 3.0 kb (3 kb Tg) or 1.0 kb (1 kb Tg) of the Runx2 distal (P1) promoter, Runx2 <jats:italic>LacZ<\/jats:italic> knock\u2010in (Runx2<jats:sup>+\/LacZ<\/jats:sup>) and Runx2\/P1 <jats:italic>LacZ<\/jats:italic> knock\u2010in (Runx2\/P1<jats:sup>+\/LacZ<\/jats:sup>). In the Runx2 3 kb Tg mouse, \u03b2\u2010galactosidase (\u03b2\u2010gal) expression appeared in various non\u2010skeletal tissues including testis, skin, adrenal gland and brain. \u03b2\u2010gal expression from both 3 kb and 1 kb Tg, reflecting activity of the Runx2 promoter, was readily detectable in seminiferous tubules of the testis and the epididymis. At the single cell level, \u03b2\u2010gal was detected in spermatids and mature sperms not in sertoli or Leydig cells. We also detected a positive signal from the Runx2<jats:sup>+\/LacZ<\/jats:sup> and Runx2\/P1<jats:sup>+\/LacZ<\/jats:sup> mice. Indeed, Runx2 expression was observed in isolated mature sperms, which was confirmed by RT\u2010PCR and Western blot analysis. Runx2, however, was not related to sex determination and sperm motility. Runx2 mediated \u03b2\u2010gal activity is also found robustly in the hippocampus and frontal lobe of the brain in Runx2<jats:sup>+\/LacZ<\/jats:sup>. Collectively, these results indicate that Runx2 is expressed in several non\u2010skeletal tissues particularly sperms of testis and hippocampus of brain. It suggests that Runx2 may play an important role in male reproductive organ testis and brain. J. Cell. Physiol. 217: 511\u2013517, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21524","type":"journal-article","created":{"date-parts":[[2008,7,17]],"date-time":"2008-07-17T18:12:55Z","timestamp":1216318375000},"page":"511-517","source":"Crossref","is-referenced-by-count":71,"title":["Expression of Runx2 transcription factor in non\u2010skeletal tissues, sperm and brain"],"prefix":"10.1002","volume":"217","author":[{"given":"Jae\u2010Hwan","family":"Jeong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung\u2010Sook","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun\u2010Nam","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010Min","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie C.","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Lengner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Otto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Mundlos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet L.","family":"Stein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre J.","family":"van Wijnen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane B.","family":"Lian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary S.","family":"Stein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je\u2010Yong","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,7,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.9.8367"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19970701)66:1<1::AID-JCB1>3.0.CO;2-V"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703806104"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.9.4892"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200290000691"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151236498"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2002.60"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4652(200009)184:3<341::AID-JCP8>3.0.CO;2-Z"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80257-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2660(08)60394-6"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20909"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413665200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01544-06"},{"key":"e_1_2_6_15_1","first-page":"234","volume-title":"Gene targeting: A practical approach","author":"Joyner AL","year":"2000"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2007.03.012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2006.11.020"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20552"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2006.05.005"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10038-004-0139-2"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2741\/2730"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20958"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80258-5"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2003.07.001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0797-307"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.23.8783-8792.2000"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502267200"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00050-3"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukaryotGeneExpr.v14.i12.10"},{"key":"e_1_2_6_30_1","unstructured":"LiuJ LengnerC GaurT HendersonJ van WijnenA SteinJ SteinG LianJ.2007.Developmentally selective expression and activities of runx2 isoforms in vivo. 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