{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:35:40Z","timestamp":1790847340026,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,9,19]],"date-time":"2008-09-19T00:00:00Z","timestamp":1221782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteogenic supplements are a requirement for osteoblastic cell differentiation during in vitro culture of human calvarial suture\u2010derived cell populations. We investigated the ability of ascorbic acid and \u03b2\u2010glycerophosphate with and without the addition of dexamethasone to stimulate in vivo\u2010like osteoblastic differentiation. Cells were isolated from unfused and prematurely fused suture tissue from patients with syndromic and non\u2010syndromic craniosynostosis and cultured in each osteogenic medium for varying lengths of time. The effect of media supplementation was investigated with respect to the ability of cells to form mineralised bone nodules and the expression of five osteodifferentiation marker genes (<jats:italic>COL1A1<\/jats:italic>, <jats:italic>ALP<\/jats:italic>, <jats:italic>BSP<\/jats:italic>, <jats:italic>OC<\/jats:italic> and <jats:italic>RUNX2<\/jats:italic>), and five genes that are differentially expressed during human premature suture fusion (<jats:italic>GPC3<\/jats:italic>, <jats:italic>RBP4<\/jats:italic>, <jats:italic>C1QTNF3<\/jats:italic>, <jats:italic>WIF1<\/jats:italic> and <jats:italic>FGF2<\/jats:italic>). Cells from unfused sutures responded more slowly to osteogenic media but formed comparable bone nodules to fused suture\u2010derived cells after 16 days of culture in either osteogenic media. However, gene expression differed between unfused and fused suture\u2010derived cells, as did expression in each osteogenic medium. When compared to expression in the explant tissue of origin, neither medium induced a level or profile of gene expression similar to that seen in vivo. Overall, our results demonstrate that cells from the same suture that are isolated during different stages of morphogenesis in vivo, despite being de\u2010differentiated to a similar level in vitro, respond uniquely and differently to each osteogenic medium. Further, we suggest that neither cell culture medium recapitulates differentiation via activation of the same genetic cascades as occurs in vivo. J. Cell. Physiol. 218: 183\u2013191, 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21586","type":"journal-article","created":{"date-parts":[[2008,9,19]],"date-time":"2008-09-19T14:08:21Z","timestamp":1221833301000},"page":"183-191","source":"Crossref","is-referenced-by-count":13,"title":["In vitro differentiation of human calvarial suture derived cells with and without dexamethasone does not induce in vivo\u2010like expression"],"prefix":"10.1002","volume":"218","author":[{"given":"Anna K.","family":"Coussens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian P.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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