{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T04:42:31Z","timestamp":1718426551313},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T00:00:00Z","timestamp":1236902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The three basic DNA\u2010binding domain mutations of the <jats:italic>microphthalmia<\/jats:italic>\u2010associated transcription factor (Mitf), Mitf<jats:sup>mi\/mi<\/jats:sup>, Mitf<jats:sup>or\/or<\/jats:sup>, and Mitf<jats:sup>wh\/wh<\/jats:sup> affect osteoclast differentiation with variable penetrance while completely impairing melanocyte development. <jats:italic>Mitf<\/jats:italic><jats:italic><jats:sup>or\/or<\/jats:sup><\/jats:italic> mice exhibit osteopetrosis that improves with age and their osteoclasts form functional multinuclear osteoclasts, raising the question as to why the Mitf<jats:sup>or\/or<\/jats:sup> mutation results in osteopetrosis. Here we show that <jats:italic>Mitf<\/jats:italic><jats:italic><jats:sup>or\/or<\/jats:sup><\/jats:italic> osteoclasts express normal levels of acid phosphatase 5 (<jats:italic>Acp5<\/jats:italic>) mRNA and significantly lower levels of <jats:italic>Cathepsin K<\/jats:italic> (<jats:italic>Ctsk<\/jats:italic>) mRNA during receptor activator of nuclear factor kappa B (NF\u03baB) ligand (RANKL)\u2010mediated differentiation. Studies using chromatin immunoprecipitation (ChIP) analysis indicate that low levels of Mitf<jats:sup>or\/or<\/jats:sup> protein are recruited to the <jats:italic>Ctsk<\/jats:italic> promoter. However, enrichment of Mitf\u2010transcriptional co\u2010activators PU.1 and Brahma\u2010related gene 1 (Brg1) are severely impaired at the <jats:italic>Ctsk<\/jats:italic> promoter of <jats:italic>Mitf<\/jats:italic><jats:italic><jats:sup>or\/or<\/jats:sup><\/jats:italic> osteoclast precursors, indicating that defective recruitment of co\u2010activators by the mutant Mitf<jats:sup>or\/or<\/jats:sup> results in impaired <jats:italic>Ctsk<\/jats:italic> expression in osteoclasts. Cathepsin K may thus represent a unique class of Mitf\u2010regulated osteoclast\u2010specific genes that are important for osteoclast function. J. Cell. Physiol. 220: 230\u2013237, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21755","type":"journal-article","created":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T12:34:58Z","timestamp":1236947698000},"page":"230-237","source":"Crossref","is-referenced-by-count":8,"title":["Defective co\u2010activator recruitment in osteoclasts from <i>microphthalmia\u2010oak ridge<\/i> mutant mice"],"prefix":"10.1002","volume":"220","author":[{"given":"Sudarshana M.","family":"Sharma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Said","family":"Sif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Ostrowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uma","family":"Sankar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,3,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.12.6930"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.1.103"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1402.084"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.10.1654"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1093\/genetics\/155.1.291","article-title":"Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus","volume":"155","author":"Hallsson JH","year":"2000","journal-title":"Genetics"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.10.3151"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.22.2770"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01839-06"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.3.451"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106418200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111696200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.71.2.304"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a108657"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608572200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(00)89143-X"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524023362401"},{"key":"e_1_2_6_19_1","first-page":"1871","article-title":"Mild osteopetrosis in the microphthalmia\u2010oak ridge mouse. 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