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This study reports for the first time purification to apparent homogeneity of a motility initiating protein (MIP) from epididymal plasma and its characterization using the caprine sperm model. It is a 125\u2009kDa (approximately) dimeric protein made up of two subunits: 70 and 54\u2009kDa. MIP is an acidic protein with an isoelectric point of 4.75. The motility protein at 30\u2009\u00b5g\/ml (240\u2009nM) level showed nearly maximal motility\u2010promoting activity. MIP is heat stable and it is maximally active at pH 8. It is a glycoprotein that binds with high affinity to concanavalin A and it contains mannose, galactose, and <jats:italic>N<\/jats:italic>\u2010acetyl glucosamine approximately in the ratios of 6:1:6. It is sensitive to the actions of \u03b1\u2010mannosidase and \u03b2\u2010<jats:italic>N<\/jats:italic>\u2010acetylglucoseaminidase thereby demonstrating that the sugar side chain of the glycoprotein is essential for its biological activity. Epididymal plasma is its richest source. It is also capable of enhancing forward motility of mature cauda\u2010sperm. Its antibody markedly inhibits sperm motility. MIP antibody is highly immunospecific and it recognizes both the subunits. MIP causes significant increase of the intrasperm level of cyclic AMP. MIP: the physiological motility\u2010activating protein has potential for use as a contraceptive vaccine and for solving some of the problems of human infertility and animal breeding. J. Cell. Physiol. 222:254\u2013263, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.21947","type":"journal-article","created":{"date-parts":[[2009,9,30]],"date-time":"2009-09-30T17:47:43Z","timestamp":1254332863000},"page":"254-263","source":"Crossref","is-referenced-by-count":18,"title":["Purification and characterization of a motility initiating protein from caprine epididymal plasma"],"prefix":"10.1002","volume":"222","author":[{"given":"Bijay Shankar","family":"Jaiswal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaushik","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudipta","family":"Saha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandhya Rekha","family":"Dungdung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gopal C.","family":"Majumder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,9,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00893a021"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"6744","DOI":"10.1016\/S0021-9258(17)37981-4","article-title":"Bovine sperm forward motility protein. 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