{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T04:20:59Z","timestamp":1786767659595,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,6]],"date-time":"2010-01-06T00:00:00Z","timestamp":1262736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MUC4 is a heterodimeric membrane mucin, composed of a mucin subunit ASGP\u20101 (MUC4\u03b1) and a transmembrane subunit ASGP\u20102 (MUC4\u03b2), which has been implicated in the protection of epithelial cell surfaces. In the rat stratified corneal epithelium Muc4 is found predominantly in the most superficial cell layers. Since previous studies in other tissues have shown that Muc4 is regulated by TGF\u2010\u03b2 via a proteosomal degradation mechanism, we investigated the regulation of corneal Muc4 in stratified cultures of corneal epithelial cells. Application of proteosome or processing inhibitors led to increases in levels of Muc4, particularly in the basal and intermediate levels of the stratified cultures. These changes were accompanied by increases in Muc4 ubiquitination, chaperone association and incorporation into intracellular aggresomes. In contrast, treatment with TGF\u2010\u03b2 resulted in reduced levels of Muc4, which were reversed by proteosome inhibition. The results support a model in which Muc4 precursor is synthesized in all layers of the corneal epithelium, but Muc4 is degraded in basal and intermediate layers by a proteosomal mechanism at least partly dependent on TGF\u2010\u03b2 inhibition of Muc4 processing. J. Cell. Physiol. 223: 209\u2013214, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22028","type":"journal-article","created":{"date-parts":[[2010,1,6]],"date-time":"2010-01-06T16:16:11Z","timestamp":1262794571000},"page":"209-214","source":"Crossref","is-referenced-by-count":11,"title":["Regulation of the membrane mucin Muc4 in corneal epithelial cells by proteosomal degradation and TGF\u2010\u03b2"],"prefix":"10.1002","volume":"223","author":[{"given":"Joseph","family":"Lomako","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wieslawa M.","family":"Lomako","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie A.","family":"Carothers Carraway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kermit L.","family":"Carraway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,1,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5263"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(02)71043-X"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(03)00204-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4652(200011)185:2<310::AID-JCP16>3.0.CO;2-W"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205970"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.52.33245"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020862"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20101"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22177"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199807)176:1<110::AID-JCP13>3.0.CO;2-B"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3300737"},{"key":"e_1_2_5_13_1","first-page":"1316","article-title":"Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid","volume":"41","author":"Pflugfelder SC","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.52.35228"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3350457"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.23.17800"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303220200"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-09-0895"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.52.33476"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240400406"},{"key":"e_1_2_5_21_1","doi-asserted-by":"crossref","first-page":"8505","DOI":"10.1016\/S0021-9258(19)38917-3","article-title":"Biosynthesis of the cell surface sialomucin complex of ascites 13762 rat mammary adenocarcinoma cells from a high molecular weight precursor","volume":"265","author":"Sheng ZQ","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_5_22_1","doi-asserted-by":"crossref","first-page":"16341","DOI":"10.1016\/S0021-9258(18)42007-8","article-title":"Molecular cloning of the transmembrane component of the 13762 mammary adenocarcinoma sialomucin complex. 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