{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T05:55:16Z","timestamp":1773726916255,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,4,2]],"date-time":"2010-04-02T00:00:00Z","timestamp":1270166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Collecting ducts (CD) not only constitute the final site for regulating urine concentration by increasing apical membrane Aquaporin\u20102 (AQP2) expression, but are also essential for the control of acid\u2013base status. The aim of this work was to examine, in renal cells, the effects of chronic alkalosis on cell growth\/death as well as to define whether AQP2 expression plays any role during this adaptation. Two CD cell lines were used: WT\u2010 (not expressing AQPs) and AQP2\u2010RCCD<jats:sub>1<\/jats:sub> (expressing apical AQP2). Our results showed that AQP2 expression per se accelerates cell proliferation by an increase in cell cycle progression. Chronic alkalosis induced, in both cells lines, a time\u2010dependent reduction in cell growth. Even more, cell cycle movement, assessed by 5\u2010bromodeoxyuridine pulse\u2010chase and propidium iodide analyses, revealed a G2\/M phase cell accumulation associated with longer S\u2010 and G2\/M\u2010transit times. This G2\/M arrest is paralleled with changes consistent with apoptosis. All these effects appeared 24\u2009h before and were always more pronounced in cells expressing AQP2. Moreover, in AQP2\u2010expressing cells, part of the observed alkalosis cell growth decrease is explained by AQP2 protein down\u2010regulation. We conclude that in CD cells alkalosis causes a reduction in cell growth by cell cycle delay that triggers apoptosis as an adaptive reaction to this environment stress. Since cell volume changes are prerequisite for the initiation of cell proliferation or apoptosis, we propose that AQP2 expression facilitates cell swelling or shrinkage leading to the activation of channels necessary to the control of these processes. J. Cell. Physiol. 224: 405\u2013413, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22136","type":"journal-article","created":{"date-parts":[[2010,4,2]],"date-time":"2010-04-02T17:39:31Z","timestamp":1270229971000},"page":"405-413","source":"Crossref","is-referenced-by-count":18,"title":["Adaptation to alkalosis induces cell cycle delay and apoptosis in cortical collecting duct cells: Role of 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