{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T01:39:09Z","timestamp":1768786749578,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,4,28]],"date-time":"2010-04-28T00:00:00Z","timestamp":1272412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective was to study Dupuytren's myofibroblast cells in constrained collagen matrices in order to more closely emulate their in vivo environment and, to correlate their contractility with \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) expression and determine if dermal fibroblasts regulate Dupuytren's myofibroblast phenotype. Isotonic and isometric force contraction by cells isolated from Dupuytren's nodules, palmar and non\u2010palmar skin fibroblasts was measured in collagen matrices. The effect of co\u2010culturing nodule cells with dermal fibroblasts on isometric contraction was examined. Isometric contraction was correlated with levels of \u03b1\u2010SMA mRNA by pcr and protein by Western blotting, and \u03b1\u2010SMA distribution assessed by immunofluorescence. Dupuytren's nodule cells exhibited similar levels of isotonic contraction to both palmar and non\u2010palmar dermal fibroblasts. However, nodule cells generated high levels of isometric force (mean: 3.5\u2009dynes\/h), which continued to increase over 24\u2009h to a maximum of 173\u2009dynes. In contrast, dermal fibroblasts initially exhibited low levels of contraction (mean: 0.5\u2009dynes\/h) and reached tensional homeostasis on average after 15\u2009h (range: 4\u201320\u2009h), with a maximum force of 52\u2009dynes. Although all three cell types had similar \u03b1\u2010SMA mRNA levels, increased levels of \u03b1\u2010SMA protein were observed in nodule cells compared to dermal fibroblasts. \u03b1\u2010SMA localised to stress fibres in 35% (range: 26\u201350%) of nodule cells compared to only 3% (range:0\u20136%) of dermal fibroblasts. Co\u2010cultures of Dupuytren's cells and dermal fibroblasts showed no contractile differences. The contractile phenotype of Dupuytren's myofibroblasts is determined by increased \u03b1\u2010SMA protein distributed in stress fibres, not by cellular mRNA levels. Dupuytren's cell contractility is not influenced by dermal fibroblasts. J. Cell. Physiol. 224: 681\u2013690, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22167","type":"journal-article","created":{"date-parts":[[2010,4,29]],"date-time":"2010-04-29T20:46:16Z","timestamp":1272573976000},"page":"681-690","source":"Crossref","is-referenced-by-count":23,"title":["Post\u2010transcriptional regulation of \u03b1\u2010smooth muscle actin determines the contractile phenotype of Dupuytren's nodular cells"],"prefix":"10.1002","volume":"224","author":[{"given":"Liaquat Suleman","family":"Verjee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Midwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Eastwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jagdeep","family":"Nanchahal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65334-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1274"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970110405"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0266-7681(03)00135-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000101527.76293.F1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198163050-00014"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1226(94)90075-2"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199806)175:3<323::AID-JCP10>3.0.CO;2-6"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0363-5023(78)80112-9"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0266-7681(03)00221-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/exmp.2001.2385"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.1.103"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.18.3285"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(94)90040-X"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0169(1998)40:1<13::AID-CM2>3.0.CO;2-G"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199601)166:1<33::AID-JCP4>3.0.CO;2-H"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.024521"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-9053(05)80271-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200506179"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.4.401"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(03)00057-6"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0266-7681(97)80061-7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700613"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.9.2730"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61776-2"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-196106000-00001"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0266-7681(05)80273-6"},{"key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1302\/0301-620X.45B4.709","article-title":"The genetic factor in Dupuytren's disease","volume":"45","author":"Ling RS","year":"1963","journal-title":"J Bone Joint Surg Br"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.2106\/00004623-195941040-00008","article-title":"Dupuytren's contracture; 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