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In this study, we observed that BNP increased the tetraethylammonium chloride (TEA)\u2010sensitive delayed rectifier outward potassium current (I<jats:sub>K<\/jats:sub>) in mouse Schwann cells (SCs) using whole\u2010cell recording techniques. At concentrations of 1\u2013100\u2009nM, BNP reversibly activated I<jats:sub>K<\/jats:sub>in a dose\u2010dependent manner, with modulating its steady\u2010state activation and inactivation properties. The effect of BNP on I<jats:sub>K<\/jats:sub>was abolished by preincubation with the specific antagonist of NPR\u2010A, and could not be mimicked by application of NPR\u2010C agonist. These results were supported by immunocytochemical findings indicating that NPR\u2010A was expressed in SCs. The application of 8\u2010Br\u2010guanosine 3\u2032,5\u2032\u2010monophosphate (8\u2010Br\u2010cGMP) mimicked the effect of BNP on I<jats:sub>K<\/jats:sub>, but BNP was unable to further increase I<jats:sub>K<\/jats:sub>after the application of cyclic guanosine monophosphate (cGMP). Genistein blocked I<jats:sub>K<\/jats:sub>and also completely eliminated the effects of BNP and cGMP on I<jats:sub>K<\/jats:sub>. The selective K<jats:sub>V<\/jats:sub>2.1 subunit blocker, Jingzhaotoxin\u2010III (JZTX\u2010III), reduced I<jats:sub>K<\/jats:sub>amplitude by 30%, but did not abolish the increase effect of BNP on I<jats:sub>K<\/jats:sub>amplitude. In addition, BNP significantly stimulated SCs proliferation and this effect could be partly inhibited by TEA. Together these results suggest that BNP modulated I<jats:sub>K<\/jats:sub>probably via cGMP\u2010 and tyrosine kinase\u2010dependent pathways by activation of NPR\u2010A. This effect of BNP on I<jats:sub>K<\/jats:sub>in SCs might partly explain its effect on cell proliferation. J. Cell. Physiol. 226: 440\u2013449, 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22352","type":"journal-article","created":{"date-parts":[[2010,7,29]],"date-time":"2010-07-29T15:00:37Z","timestamp":1280415637000},"page":"440-449","source":"Crossref","is-referenced-by-count":3,"title":["Brain natriuretic peptide modulates the delayed rectifier outward K<sup>+<\/sup>current and promotes the proliferation of mouse schwann 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