{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T15:15:13Z","timestamp":1775834113013,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,8,24]],"date-time":"2011-08-24T00:00:00Z","timestamp":1314144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Jab1, a co\u2010activator of AP\u20101 transcription factor and the fifth subunit of the COP9 signalosome, mediates degradation of the tumor suppressor p53 and p27<jats:sup>Kip1<\/jats:sup> and functions as a tumor promoter in different types of human cancer. In this study, we show that inhibition of Bcr\u2010Abl oncogene by imatinib induces down\u2010regulation of Jab1 in Bcr\u2010Abl\u2010positive K562, Ku812, and MEG01 leukemia cells suggesting Bcr\u2010Abl may regulate Jab1 expression. Promoter deletion and mutation analysis indicate the Tcf\u20104\/\u03b2\u2010catenin and STAT1 binding sites located between the \u2212405\/\u2212223 region of the human Jab1 promoter are important for the activation of Jab1 by Bcr\u2010Abl. Double mutation of these two sites reverses the inhibitory effect of imatinib. Chromatin immunoprecipitation assay verifies the binding of \u03b2\u2010catenin and STAT1 to human Jab1 promoter. Ectopic expression of dominant\u2010negative Tcf\u20104 mutant significantly attenuates Jab1 expression while over\u2010expression of \u03b2\u2010catenin and STAT1 cooperatively up\u2010regulates Jab1 promoter activity and mRNA expression. Our results also demonstrate that the AKT signaling pathway is involved in the regulation of Jab1 by Bcr\u2010Abl because the AKT inhibitor LY294002 but not the ERK inhibitor PD98059 reduces Jab1 promoter activity and mRNA expression. Taken together, our results suggest that Bcr\u2010Abl stimulates Jab1 expression via the cooperative interaction of \u03b2\u2010catenin and STAT1 in leukemia cells. J. Cell. Physiol. 226: 2849\u20132856, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22633","type":"journal-article","created":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T14:30:27Z","timestamp":1296570627000},"page":"2849-2856","source":"Crossref","is-referenced-by-count":8,"title":["Bcr\u2010Abl oncogene stimulates jab1 expression via cooperative interaction of \u03b2\u2010catenin and STAT1 in chronic myeloid leukemia cells"],"prefix":"10.1002","volume":"226","author":[{"given":"Kuei\u2010Ting","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Chung","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Yi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Chuan","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Chun","family":"Hung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,8,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100442200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2008.11.023"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.811"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-06-0812"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2009.03.007"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22346"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/383453a0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204064"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.22.3499"},{"key":"e_1_2_6_11_1","first-page":"5652","article-title":"Jun activation domain binding protein 1 expression is associated with low p27(Kip1)levels in node\u2010negative breast cancer","volume":"9","author":"Esteva FJ","year":"2003","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_12_1","first-page":"1724","article-title":"BCR\/abl leads to the constitutive activation of Stat proteins, and shares an epitope with tyrosine phosphorylated Stats","volume":"10","author":"Frank DA","year":"1996","journal-title":"Leukemia"},{"key":"e_1_2_6_13_1","first-page":"277","article-title":"Prognostic significance of localized p27Kip1 and potential role of Jab1\/CSN5 in pancreatic cancer","volume":"11","author":"Fukumoto A","year":"2004","journal-title":"Oncol Rep"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510937200"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1677\/ERC-07-0077"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-5040"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1182\/blood.V96.5.1933","article-title":"Bcr\u2010Abl kinase down\u2010regulates cyclin\u2010dependent kinase inhibitor p27 in human and murine cell lines","volume":"96","author":"Jonulet T","year":"2000","journal-title":"Blood"},{"key":"e_1_2_6_18_1","first-page":"2977","article-title":"Jun activation domain\u2010binding protein 1 expression in breast cancer inversely correlates with the cell cycle inhibitor p27(Kip1)","volume":"63","author":"Kouvaraki MA","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.2.4.433"},{"key":"e_1_2_6_20_1","first-page":"3069","article-title":"Histone deacetylase inhibitor up\u2010regulates RECK to inhibit MMP\u20102 activation and cancer cell invasion","volume":"63","author":"Liu LT","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M601857200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403999"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1567"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.6.469"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.959"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000156273.30274.f7"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406499101"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/18230"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1242"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf024"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.3.809.809"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00372-7"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.19.3259"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.22633","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.22633","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T06:19:08Z","timestamp":1696918748000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.22633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,8,24]]},"references-count":32,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1002\/jcp.22633"],"URL":"https:\/\/doi.org\/10.1002\/jcp.22633","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,8,24]]}}}