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Here, we show that endothelial cells from the oak ridge polycystic kidney (Tg737<jats:sup>orpk\/orpk<\/jats:sup>) mouse, with impaired cilia assembly, exhibit a reduction in the actin stress fibers and focal adhesions compared to wild\u2010type (WT). In contrast, endothelial cells from polycystin\u20101 deficient mice (pkd1<jats:sup>null\/null<\/jats:sup>), with impaired cilia function, display robust stress fibers, and focal adhesion assembly. We found that the Tg737<jats:sup>orpk\/orpk<\/jats:sup> cells exhibit impaired directional migration and endothelial cell monolayer permeability compared to the WT and pkd1<jats:sup>null\/null<\/jats:sup> cells. Finally, we found that the expression of heat shock protein 27 (hsp27) and the phosphorylation of focal adhesion kinase (FAK) are downregulated in the Tg737<jats:sup>orpk\/orpk<\/jats:sup> cells and overexpression of hsp27 restored both FAK phosphorylation and cell migration. Taken together, these results demonstrate that disruption of the primary cilia structure or function compromises the endothelium through the suppression of hsp27 dependent actin organization and focal adhesion formation, which may contribute to the vascular dysfunction in ciliopathies. J. Cell. Physiol. 227: 70\u201376, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22704","type":"journal-article","created":{"date-parts":[[2011,2,26]],"date-time":"2011-02-26T15:13:03Z","timestamp":1298733183000},"page":"70-76","source":"Crossref","is-referenced-by-count":66,"title":["Primary cilia regulates the directional migration and barrier integrity of endothelial cells through the modulation of Hsp27 dependent actin cytoskeletal organization"],"prefix":"10.1002","volume":"227","author":[{"given":"Thomas J.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi K.","family":"Adapala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner J.","family":"Geldenhuys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Bursley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wissam A.","family":"AbouAlaiwi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surya M.","family":"Nauli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles K.","family":"Thodeti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,10,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.192765"},{"key":"e_1_2_5_3_1","first-page":"pe6","article-title":"Mechanotransduction: All signals point to cytoskeleton, matrix, and integrins","volume":"119","author":"Alenghat FJ","year":"2002","journal-title":"Sci STKE"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.08.003"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.05.025"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)00810-7"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20946"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1681\/ASN.V121194","article-title":"Hypertension in autosomal\u2010dominant polycystic kidney disease: Early occurrence and unique aspects","volume":"12","author":"Ecder T","year":"2001","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.011209.122036"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21472"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5424rev"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R300038200"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjhyper.2004.06.020"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2004.01.011"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2741\/3016"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2008.03.006"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21773"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.07.003"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.11.2347"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00370.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1076"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.710111"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005080830"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20069"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00096.2004"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-001-0075-4"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-002-1042-4"},{"key":"e_1_2_5_29_1","unstructured":"RasbandWS.1997\u20102007.ImageJ. 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