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Here, we explored the role of senescent fibroblasts on neovascularization with a mouse model of alkali\u2010induced corneal wound healing. Senescent cells accumulated in corneal stroma from day 7 to 27 after alkali burn and peaked on day 14, which was consistent with the development of corneal neovascularization (CNV). In vitro and in vivo assays confirmed that the senescent cells were derived primarily from activated corneal fibroblasts. Furthermore, senescent corneal fibroblasts exhibited enhanced synthesis and secretion of extracellular matrix\u2010degrading enzymes (matrix metalloproteinases 2, 3, and 14 and tissue\u2010 and urokinase\u2010type plasminogen activators) and angiogenic factors (vascular endothelial growth factor) and decreased expression of anti\u2010angiogenic factors (pigment epithelium\u2010derived factor and thrombospondins), which supported the proliferation, migration, and promotion of tube formation of vascular endothelial cells. Intrastromal injection of premature senescent fibroblasts induced CNV earlier than that of normal fibroblasts, while matrix metalloproteinase inhibitors blocked the early onset of senescent cell\u2010induced CNV. Therefore, senescent fibroblasts promoted the alkali\u2010induced CNV partially via the enhanced secretion of matrix metalloproteases. J. Cell. Physiol. 227: 1148\u20131156, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22835","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T15:59:17Z","timestamp":1305215957000},"page":"1148-1156","source":"Crossref","is-referenced-by-count":22,"title":["Role of senescent fibroblasts on alkali\u2010induced corneal neovascularization"],"prefix":"10.1002","volume":"227","author":[{"given":"Qingjun","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingli","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-008-0358-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1373\/49.1.32"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713680802461106"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1716"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2233"},{"key":"e_1_2_6_7_1","first-page":"17","volume-title":"Wound healing. 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