{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T05:11:22Z","timestamp":1696828282859},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T00:00:00Z","timestamp":1326240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b2Pix, a Pak\u2010interacting nucleotide exchange factor (Cool\u20101\/p85SPR), is a Cdc42\/Rac1\u2010specific guanine nucleotide exchange factor (GEF) involved in various actin\u2010related processes. Many previous studies have focused on ubiquitously expressed \u03b2Pix\u2010a, while the role of the neuronal\u2010specific isoform \u03b2Pix\u2010b is still unknown, especially whether its role is distinct from or similar to \u03b2Pix\u2010a. Here we show that unlike \u03b2Pix\u2010a, overexpression of \u03b2Pix\u2010b stimulates actin\u2010dependent comet formation in BHK21 cells. This effect is attributed to the interaction between its proline\u2010rich domain (PRD) and the WH1 domain of N\u2010WASP. In addition, we show that overexpression of \u03b2Pix\u2010b stimulates actin\u2010dependent dendritic spine formation in rat hippocampal neurons in culture, a formation that is blocked by co\u2010expression of the WH1 domain of N\u2010WASP or the PRD of \u03b2Pix\u2010b. Knocking\u2010down endogenous expression of \u03b2Pix\u2010b by shRNA reduced the number of dendritic spines, which were rescued only by PRD\u2010containing \u03b2Pix\u2010b mutants. GEF activity of \u03b2Pix\u2010b is also required for these effects. The results show that neuronal\u2010specific \u03b2Pix\u2010b stimulates actin\u2010dependent processes in cells via the interaction between its PRD and the WH1 domain of N\u2010WASP. Our results identify N\u2010WASP as the first protein shown to interact with the PRD of \u03b2Pix\u2010b, raising the possibility that, as an N\u2010WASP WH1\u2010binding protein, \u03b2Pix\u2010b may regulate N\u2010WASP's activity in cells. J. Cell. Physiol. 227: 1476\u20131484, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22863","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T13:03:04Z","timestamp":1306414984000},"page":"1476-1484","source":"Crossref","is-referenced-by-count":3,"title":["Neuronal specific \u03b2Pix\u2010b stimulates actin\u2010dependent processes via the interaction between its PRD and WH1 domain of N\u2010WASP"],"prefix":"10.1002","volume":"227","author":[{"given":"Joohyun","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoonju","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zee Yong","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongeun","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunghoe","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00433"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/90489"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.06.027"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201003008"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn964"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2845"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000806200"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.23.4239"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601349"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3112-08.2008"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310974200"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80019-2"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/35074551"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/9048"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvg146"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/35017080"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6875"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301052200"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.29.22373"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.04.039"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00384-5"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.11.016"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209329"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.11.1905"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1063(2000)10:5<501::AID-HIPO1>3.0.CO;2-T"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.3.519"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801555200"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02996"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.17.6354-6363.2000"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.22863","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.22863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T19:40:37Z","timestamp":1696794037000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.22863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,11]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/jcp.22863"],"URL":"https:\/\/doi.org\/10.1002\/jcp.22863","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,11]]}}}