{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:54:52Z","timestamp":1762458892077},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,1,11]],"date-time":"2012-01-11T00:00:00Z","timestamp":1326240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated whether transplantation of purified human peripheral blood CD34<jats:sup>+<\/jats:sup> cells could reduce established liver fibrosis and up\u2010regulate therapeutic regeneration. Human peripheral blood CD34<jats:sup>+<\/jats:sup> cells were isolated from total mononuclear cells of healthy volunteers by magnetic cell sorting. Recipient nude rats were injected intraperitoneally with carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) twice weekly for 3 weeks before single administration of CD34<jats:sup>+<\/jats:sup> cells. CCl<jats:sub>4<\/jats:sub> was then re\u2010administered twice weekly for 3 more weeks, and the nude rats were sacrificed. Saline (control group), 1\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> (low\u2010dose group), 5\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> (middle\u2010dose group), or 2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> (high\u2010dose group) CD34<jats:sup>+<\/jats:sup> cells\/kg body weight were intrasplenically transplanted after CCl<jats:sub>4<\/jats:sub> treatment for 3 weeks. Reverse transcriptase\u2010polymerase chain reaction analysis of the freshly isolated CD34<jats:sup>+<\/jats:sup> cells revealed the expression of CD31, keratin19, \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), and epithelial growth factor, but not other liver related markers. The transplanted cells differentiated into vascular and sinusoidal endothelial cells, and vascular smooth muscle cells. CD34<jats:sup>+<\/jats:sup> cell transplantation reduced liver fibrosis in a dose\u2010dependent fashion, with decreased collagen type\u2010I and \u03b1\u2010SMA\u2010positive cells after 6 weeks of CCl<jats:sub>4<\/jats:sub> treatment by Mallory's Azan and immunohistochemical staining. Gelatin zymography showed that the expression levels of active matrix metalloproteinase\u20102 and \u20109 in CD34<jats:sup>+<\/jats:sup> cell transplanted livers were significantly stronger than those in saline\u2010infused livers. In recipients of high\u2010doses of CD34<jats:sup>+<\/jats:sup> cells, the number of PCNA\u2010positive hepatocyte increased 6 weeks after CCl<jats:sub>4<\/jats:sub> treatment compared with saline\u2010infused livers. We conclude that human peripheral blood CD34<jats:sup>+<\/jats:sup> cell transplantation halts established liver fibrosis and promotes hepatic regeneration in CCl<jats:sub>4<\/jats:sub>\u2010induced chronic liver injury. J. Cell. Physiol. 227: 1538\u20131552, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22873","type":"journal-article","created":{"date-parts":[[2011,6,15]],"date-time":"2011-06-15T14:55:16Z","timestamp":1308149716000},"page":"1538-1552","source":"Crossref","is-referenced-by-count":19,"title":["Human peripheral blood CD34\u2010positive cells enhance therapeutic regeneration of chronically injured liver in nude 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