{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:08:40Z","timestamp":1762160920122},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,1,23]],"date-time":"2012-01-23T00:00:00Z","timestamp":1327276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transplantation of cultivated human limbo\u2010corneal epithelial (HLE) cells has been recognized as an effective stem cell (SC) therapy for treating corneal epithelial SC deficiency caused by burn or other diseases. With this technique, cryo\u2010preserved human intact amniotic membrane (IAM) has been successfully used as a cell culture substrate and carrier, and is reported to preferentially preserve HLE stem\/progenitor cells in vitro. However, little is known about what factors released by HLE cells are involved in the progenitor cell\u2010preserving mechanism. Using proteomic method, we identified 13 proteins over\u2010expressed by HLE cells cultured on IAM, which included heat shock protein 70\u20101 (Hsp70\u20101), Hsp\u201027, glutathione (GSH) S\u2010transferase, annexin A2, galectin\u20107, and protein S100\u2010A9. Increased Hsp70\u20101 expression was confirmed by Western blot and real\u2010time PCR. The role of Hsp70\u20101 in promoting HLE cell survival was demonstrated by increased apoptosis index and increased cleaved poly ADP\u2010ribose polymerase (CPARP) formation in <jats:italic>hsp70\u20101<\/jats:italic>\u2010silenced, but not normal HLE cells after exposure to sublethal UVB irradiation or hydrogen peroxide. To understand the regulatory mechanism of Hsp70\u20101 expression in HLE cells, the role of transcription factor deltaNp63 (a well\u2010recognized HLE stem cell; SC marker) was studied. We found that over\u2010expression of deltaNp63\u03b1 by plasmid vector induced a corresponding increase in Hsp70\u20101 protein production. Likewise, Hsp70\u20101 expression decreased in HLE cells after addition of deltaNp63\u03b1 SiRNA. Immunoconfocal microscopy also revealed a paralleled expression of both proteins in corneal specimens. Thus, deltaNp63\u03b1\u2010associated Hsp70\u20101 over\u2010expression may promote HLE progenitor cell survival on IAM, possibly through the cytoprotective and anti\u2010apoptotic effect of Hsp70\u20101. J. Cell. Physiol. 227: 2030\u20132039, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcp.22932","type":"journal-article","created":{"date-parts":[[2011,7,12]],"date-time":"2011-07-12T20:53:39Z","timestamp":1310504019000},"page":"2030-2039","source":"Crossref","is-referenced-by-count":14,"title":["Up\u2010regulation of heat shock protein 70\u20101 (Hsp70\u20101) in human limbo\u2010corneal epithelial cells cultivated on amniotic membrane: A proteomic study"],"prefix":"10.1002","volume":"227","author":[{"given":"David Hui\u2010Kang","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jui\u2010Yang","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szu\u2010Tzu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Ye","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Unique","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung\u2010Chi Jesse","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lung\u2010Kung","family":"Yeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Ju","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Fang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan\u2010Kan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2010Kuo","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3791"},{"key":"e_1_2_6_3_1","first-page":"1711","article-title":"Proteomic analysis of epithelium\u2010denuded human amniotic membrane as a limbal stem cell niche","volume":"13","author":"Baharvand H","year":"2007","journal-title":"Mol Vis"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgl152"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3377"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.05.039"},{"key":"e_1_2_6_7_1","first-page":"2952","article-title":"Constitutive and inducible heat shock protein 70 immunoreactivity in the normal rat eye","volume":"40","author":"Dean DO","year":"1999","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_8_1","first-page":"3430","article-title":"UVB\u2010mediated induction of interleukin\u20106 and \u20108 in pterygia and cultured human pterygium epithelial cells","volume":"43","author":"Di GN","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_9_1","first-page":"2031","article-title":"Preferential gene expression in the limbus of the vervet monkey","volume":"14","author":"Ding Z","year":"2008","journal-title":"Mol Vis"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.8.3264"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0346"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000187624"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5427"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.22.3448"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.120.6.783"},{"key":"e_1_2_6_16_1","first-page":"63","article-title":"Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane","volume":"43","author":"Grueterich M","year":"2002","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(02)01105-3"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2003.08.003"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2003.08.003"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0943"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00596.2002"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0776"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10273"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.2.899-911.2004"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.03.009"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(01)00694-7"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00156-6"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/hs1201.099604"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7310137"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0591"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.05.001"},{"key":"e_1_2_6_32_1","first-page":"878","article-title":"Conjunctival epithelial cell differentiation on amniotic membrane","volume":"40","author":"Meller D","year":"1999","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.86.4.463"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/19531"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600446"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040455"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63850-3"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004030000173"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)11188-0"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1997.01100160530001"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200111150-00002"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0905955"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.08.020"},{"key":"e_1_2_6_44_1","first-page":"8233","article-title":"Chemosensitization by a non\u2010apoptogenic heat shock protein 70\u2010binding apoptosis\u2010inducing factor mutant","volume":"63","author":"Schmitt E","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000224643.43601.5d"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(02)01089-8"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0580"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00466.2005"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70119-2"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0590"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj477"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1463206"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007133430202"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.116.4.431"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00310-2"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601375"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0272"},{"key":"e_1_2_6_58_1","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1158\/0008-5472.758.65.3","article-title":"DeltaNp63alpha up\u2010regulates the Hsp70 gene in human cancer","volume":"65","author":"Wu G","year":"2005","journal-title":"Cancer Res"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512655200"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/35043127"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/19539"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(02)02595-7"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.22932","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.22932","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T20:52:14Z","timestamp":1696798334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.22932"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,23]]},"references-count":61,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/jcp.22932"],"URL":"https:\/\/doi.org\/10.1002\/jcp.22932","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1,23]]}}}