{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:11:03Z","timestamp":1790863863764,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T00:00:00Z","timestamp":1330041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    CD731 is a GPI\u2010anchored cell surface protein with ecto\u20105\u2032\u2010nucleotidase enzyme activity that plays a crucial role in adenosine production. While the roles of adenosine receptors (AR) on osteoblasts and osteoclasts have been unveiled to some extent, the roles of CD73 and CD73\u2010generated adenosine in bone tissue are largely unknown. To address this issue, we first analyzed the bone phenotype of CD73\u2010deficient (\n                    <jats:italic>cd73<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice. The mutant male mice showed osteopenia, with significant decreases of osteoblastic markers. Levels of osteoclastic markers were, however, comparable to those of wild\u2010type mice. A series of in vitro studies revealed that CD73 deficiency resulted in impairment in osteoblast differentiation but not in the number of osteoblast progenitors. In addition, over expression of CD73 on MC3T3\u2010E1 cells resulted in enhanced osteoblastic differentiation. Moreover, MC3T3\u2010E1 cells expressed adenosine A\n                    <jats:sub>2A<\/jats:sub>\n                    receptors (A\n                    <jats:sub>2A<\/jats:sub>\n                    AR) and A\n                    <jats:sub>2B<\/jats:sub>\n                    receptors (A\n                    <jats:sub>2B<\/jats:sub>\n                    AR) and expression of these receptors increased with osteoblastic differentiation. Enhanced expression of osteocalcin (OC) and bone sialoprotein (BSP) observed in MC3T3\u2010E1 cells over expressing CD73 were suppressed by treatment with an A\n                    <jats:sub>2B<\/jats:sub>\n                    AR antagonist but not with an A\n                    <jats:sub>2A<\/jats:sub>\n                    AR antagonist. Collectively, our results indicate that CD73 generated adenosine positively regulates osteoblast differentiation via A\n                    <jats:sub>2B<\/jats:sub>\n                    AR signaling. J. Cell. Physiol. 227: 2622\u20132631, 2012. \u00a9 2011 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jcp.23001","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T02:39:11Z","timestamp":1314844751000},"page":"2622-2631","source":"Crossref","is-referenced-by-count":112,"title":["CD73\u2010generated adenosine promotes osteoblast differentiation"],"prefix":"10.1002","volume":"227","author":[{"given":"Masahide","family":"Takedachi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Oohara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda 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