{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:48:12Z","timestamp":1789674492542,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,6,21]],"date-time":"2012-06-21T00:00:00Z","timestamp":1340236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Progressive multifocal leukoencephalopathy (PML) is a severe disease of the central nervous system (CNS), caused by infection with the Polyomavirus JC virus (JCV). Because there are no known treatments or prognostic factors, we performed a long\u2010term study focusing mainly on cerebrospinal fluid (CSF) samples from PML patients to describe the virological features akin to the different forms of the disease. Twenty\u2010eight PML patients were enrolled: 10 HIV\u20101+ patients with classical PML (CPML), 9 HIV\u20101+ patients with slowly progressing or stable neurological symptoms (benign PML), 3 HIV\u20101+ asymptomatic patients, and 6 HIV\u20101\u2010negative patients. CSF, urine, and blood samples were collected at the enrollment (baseline) and every 6 months afterwards when possible. The JCV DNA and HIV\u20101 RNA loads were determined, and the JCV strains were characterized. At baseline, the mean CSF JCV load was log\u20096.0\u2009\u00b1\u20091.2\u2009copies\/ml for CPML patients, log\u20094.0\u2009\u00b1\u20091.0 copies\/ml for benign PML patients, log\u20094.2\u2009\u00b1\u20090.5 copies\/ml for asymptomatic PML patients, and log\u20095.8\u2009\u00b1\u20091.3\u2009copies\/ml for HIV\u20101\u2010negative PML patients (CPML vs. benign:\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01; CPML vs. asymptomatic:\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05; HIV\u20101 negative vs. benign:\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01). Organization of the JCV transcriptional control region (TCR) showed unusual archetype structures in two long\u2010term survival patients; the NF1 sequence was found most commonly, whereas the Sp1 binding site was the most common for both CPML patients and HIV\u20101 negative patients. Our results suggest that the JCV load in the CSF and the organization of the TCR should be considered as indicators of PML clinical outcome. J. Cell. Physiol. 227: 3511\u20133517, 2012. \u00a9 2012 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/jcp.24051","type":"journal-article","created":{"date-parts":[[2012,1,17]],"date-time":"2012-01-17T13:59:57Z","timestamp":1326808797000},"page":"3511-3517","source":"Crossref","is-referenced-by-count":36,"title":["JC virus load in cerebrospinal fluid and transcriptional control region rearrangements may predict the clinical course of progressive multifocal leukoencephalopathy"],"prefix":"10.1002","volume":"227","author":[{"given":"Serena","family":"Delbue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Elia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla","family":"Carloni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Tavazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Marchioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Carluccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Signorini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Novati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Maserati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pasquale","family":"Ferrante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.159-164.1996"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/514010"},{"key":"e_1_2_6_4_1","first-page":"200","article-title":"JC 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