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Emerging experimental evidence is disclosing a key regulatory role of microRNAs (miRNAs) in the regulation of bone homeostasis suggesting the miRNA network as potential novel target for the treatment of MM\u2010related bone disease (BD). Here, we report that miR\u201029b expression decreases progressively during human OCL differentiation in vitro. We found that lentiviral transduction of miR\u201029b into OCLs, even in the presence of MM cells, significantly impairs tartrate acid phosphatase (TRAcP) expression, lacunae generation, and collagen degradation, which are relevant hallmarks of OCL activity. Accordingly, expression of cathepsin K and metalloproteinase 9 (MMP9) as well as actin ring rearrangement were impaired in the presence of miR\u201029b. Moreover, we found that canonical targets C\u2010FOS and metalloproteinase 2 are suppressed by constitutive miR\u201029b expression which also downregulated the master OCL transcription factor, NAFTc\u20101. Overall, these data indicate that enforced expression of miR\u201029b impairs OCL differentiation and overcomes OCL activation triggered by MM cells, providing a rationale for miR\u201029b\u2010based treatment of MM\u2010related BD. J. Cell. Physiol. 228: 1506\u20131515, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcp.24306","type":"journal-article","created":{"date-parts":[[2012,12,18]],"date-time":"2012-12-18T21:27:30Z","timestamp":1355866050000},"page":"1506-1515","source":"Crossref","is-referenced-by-count":158,"title":["miR\u201029b negatively regulates human osteoclastic cell differentiation and function: Implications for the treatment of multiple myeloma\u2010related bone disease"],"prefix":"10.1002","volume":"228","author":[{"given":"Marco","family":"Rossi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rita","family":"Pitari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Amodio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Di 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