{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:43:31Z","timestamp":1789587811310,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T00:00:00Z","timestamp":1377216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>HIV\u20101 infection and replication are affected by host factors. Recent studies demonstrate that molecules from apoptotic pathways regulate HIV\u20101 replication. Therefore, studies on effects of host factors that maintain host cell survival and influence HIV\u20101 replication are critical to understanding the mechanisms of HIV\u20101 replicative cycle. Using the susceptible Jurkat cell line, CD4<jats:sup>+<\/jats:sup> T cells, and peripheral blood mononuclear cells (PBMCs), we studied the role of FLIP, an inhibitor of caspase\u20108, in HIV\u20101 production. Full length cellular FLIP (cFLIP) inhibited HIV\u20101 replication in these cells. cFLIP upregulated the expression of viral restriction factors, such as TRIM5, Apobec3G, and Bst2\/tetherin, decreased nuclear factor 1C expression and inactivated ERK and p38 induced by HIV\u20101 in Jurkat cells. cFLIP blocked the trafficking of gp120 and Gag p24 capsid protein into lipid rafts with inhibition of Tsg101 and Alix in ESCRT signaling pathway. cFLIP also promoted Bst2\/tetherin trafficking into lipid rafts. These results indicate that cFLIP may inhibit the HIV\u20101 replication cycle at multiple steps, including viral RNA release, transcription, traffic and assembly. We also found that cFLIP expression downregulated Fas expression and inactivated FADD in the Fas\u2010mediated apoptotic pathway. The inactivated FADD also inhibited HIV\u20101 replication. J. Cell. Physiol. 228: 2305\u20132313, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24397","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T14:04:45Z","timestamp":1369145085000},"page":"2305-2313","source":"Crossref","is-referenced-by-count":15,"title":["Some mechanisms of FLIP expression in inhibition of HIV\u20101 replication in Jurkat cells, CD4+ T cells and PBMCs"],"prefix":"10.1002","volume":"228","author":[{"given":"Jiying","family":"Tan","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research Food and Drug Administration Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Virology, Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics 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