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However, the effect of hypoxia can be very different from one cell type to the other. We studied the effect of hypoxia on the etoposide\u2010induced cell death in two cancer cell lines, HepG2 and A549 cells. Hypoxia decreased etoposide\u2010induced apoptosis in HepG2 cells but not in A549 cells. Here, we evidenced two pathways, known to play important roles in cancer cell resistance, that are differently affected by hypoxia in these two cell types. First, in HepG2 cells, hypoxia decreased p53 protein level and activity by acting post\u2010transcriptionally and independently of HIF\u20101. The results suggest an effect of hypoxia on p53 translation. On the other hand, in A549 cells, no effect of hypoxia was observed on p53 level. Secondly, hypoxia decreased DNA damage response in HepG2 cells while this was not the case in A549 cells. Indeed, a decrease in the phosphorylation level of CHK2 and H2AX with a decrease in ATM activity was observed. Importantly, these results evidenced that hypoxia can prevent cancer cell apoptosis by acting at different levels in the cell and that these effects are strongly cell\u2010type dependent. J. Cell. Physiol. 228: 2365\u20132376, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24409","type":"journal-article","created":{"date-parts":[[2013,5,23]],"date-time":"2013-05-23T19:34:44Z","timestamp":1369337684000},"page":"2365-2376","source":"Crossref","is-referenced-by-count":17,"title":["Differential effect of hypoxia on etoposide\u2010induced DNA damage response and p53 regulation in different cell types"],"prefix":"10.1002","volume":"228","author":[{"given":"Audrey","family":"Sermeus","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology (URBC) NARILIS, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Rebucci","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology (URBC) NARILIS, University of Namur Namur 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