{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T02:42:55Z","timestamp":1761619375266},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,11,22]],"date-time":"2013-11-22T00:00:00Z","timestamp":1385078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:label \/><jats:p>The oncogenic latent membrane protein 1 (LMP1) of Epstein\u2013Barr virus (EBV) is involved in the pathogenesis of human nasopharyngeal carcinoma (NPC) and lymphoma. We and other authors have shown earlier that LMP1 induces apoptosis and inhibits xenograft tumor growth in mice, but the mechanism underlying these processes has not been investigated so far. In the present study, we show that knockdown of LMP1 renders the EBV\u2010positive NPC cell line CG\u20101 resistant to various genotoxic drugs (cisplatin, etoposide, and adriamycin). LMP1 inhibits the expression of Cabin1, a Ca<jats:sup>2+<\/jats:sup> regulated protein shown earlier to inhibit calcineurin. Knockdown of calcineurin binding protein (Cabin1) with small hairpin RNA sensitizes CG\u20101 cells to genotoxic drugs. In contrast, LMP1 overexpression reduces Cabin1 level and renders both CG\u20101 cells and EBV\u2010negative NPC cell lines sensitive to cisplatin. The c\u2010Jun\u2010N\u2010terminal kinase (JNK) and ERK pathways are required for LMP1\u2010induced suppression of Cabin1 at the transcriptional level. Chromatin immunoprecipitation assays further confirm that the JNK\u2010activated transcription factor AP\u20101 mediates the LMP1\u2010induced down\u2010regulation of Cabin1 gene expression. LMP1 knockdown also increases the resistance of xenograph tumors to cisplatin in mice, therefore confirming the relevance of our findings in vivo. This study reveals the molecular mechanism underlying the pro\u2010apoptotic activity of LMP1 during cisplatin\u2010based NPC chemotherapy. J. Cell. Physiol. 229: 309\u2013322, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24448","type":"journal-article","created":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T06:42:40Z","timestamp":1376376160000},"page":"309-322","source":"Crossref","is-referenced-by-count":7,"title":["EBV\u2010Encoded LMP\u20101 Sensitizes Nasopharyngeal Carcinoma Cells to Genotoxic Drugs by Down\u2010Regulating Cabin1 Expression"],"prefix":"10.1002","volume":"229","author":[{"given":"Pu\u2010Yuan","family":"Chang","sequence":"first","affiliation":[{"name":"Tumor Biology Laboratory, Department of Biochemistry and Molecular Biology Chang Gung University College of Medicine Gueishan, Taoyuan Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zchong\u2010Zcho","family":"Wu","sequence":"additional","affiliation":[{"name":"Tumor Biology Laboratory, Department of Biochemistry and Molecular Biology Chang Gung University College of 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