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While many stem cell sources exist, including: mesenchymal stem cells, embryonic stem cells, and induced pluripotent stem cells, the limited cellular potency, technical difficulties, and ethical considerations associated with these make finding alternate sources a desirable goal. Periodontal ligament stem cells (PDLSCs) derived from the neural crest were induced into neural\u2010like cells using a combination of epidermal growth factor, and basic fibroblast growth factor. Morphological changes were evident in our treated group, seen under both light microscopy and scanning electron microscopy. A statistically significant increase in the expression of neuron\u2010specific \u03b2\u2010tubulin III and the neural stem\/progenitor cell marker nestin, along with positive immunohistochemical staining for glial fibrillary acidic protein, demonstrated the success of our treatment in inducing both neuronal and glial phenotypes. Positive staining for synaptophysin demonstrated neural connections and electrophysiological recordings indicated that when subjected to whole\u2010cell patch clamping, our treated cells displayed inward currents conducted through voltage\u2010gated sodium (Na<jats:sup>+<\/jats:sup>) channels. Taken together, our results indicate the success of our treatment in inducing PDLSCs to neural\u2010like cells. The ease of sourcing and expansion, their embryologic neural crest origin, and the lack of ethical implications in their use make PDLSCs an attractive source for use in neuroregenerative medicine. J. Cell. Physiol. 229: 479\u2013488, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24468","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T14:24:03Z","timestamp":1381242243000},"page":"479-488","source":"Crossref","is-referenced-by-count":41,"title":["Neurogenesis of Neural Crest\u2010Derived Periodontal Ligament Stem Cells by EGF and bFGF"],"prefix":"10.1002","volume":"229","author":[{"given":"Veronica R.","family":"Fortino","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering University of Miami Coral Gables Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u2010Shiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Miller School of Medicine University of Miami Miami Florida"},{"name":"Department of Life Science Tunghai University Taichung 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