{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:30:54Z","timestamp":1779352254279,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T00:00:00Z","timestamp":1409097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["R01 CA097318"],"award-info":[{"award-number":["R01 CA097318"]}]},{"name":"NIH","award":["R01 CA138540"],"award-info":[{"award-number":["R01 CA138540"]}]},{"DOI":"10.13039\/100001767","name":"National Foundation for Cancer Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001767","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000913","name":"James S. McDonnell Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000913","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>As a strategy to identify gene expression changes affected by human polynucleotide phosphorylase (<jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic>), we performed gene expression analysis of HeLa cells in which <jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> was overexpressed. The observed changes were then compared to those of HO\u20101 melanoma cells in which <jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> was stably knocked down. Through this analysis, 90 transcripts, which positively or negatively correlated with <jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> expression, were identified. The majority of these genes were associated with cell communication, cell cycle, and chromosomal organization gene ontology categories. For a number of these genes, the positive or negative correlations with <jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> expression were consistent with transcriptional data extracted from the TCGA (The Cancer Genome Atlas) expression datasets for colon adenocarcinoma (COAD), skin cutaneous melanoma (SKCM), ovarian serous cyst adenocarcinoma (OV), and prostate adenocarcinoma (PRAD). Further analysis comparing the gene expression changes between Ad.<jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> infected HO\u20101 melanoma cells and HeLa cells overexpressing <jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> under the control of a doxycycline\u2010inducible promoter, revealed global changes in genes involved in cell cycle and mitosis. Overall, this study provides further evidence that <jats:italic>hPNPase<jats:sup>old\u201035<\/jats:sup><\/jats:italic> is associated with global changes in cell cycle\u2010associated genes and identifies potential gene targets for future investigation. J. Cell. Physiol. 229: 1952\u20131962, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24645","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T10:52:54Z","timestamp":1397472774000},"page":"1952-1962","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of Global Changes in Gene Expression Induced by Human Polynucleotide Phosphorylase (<i>hPNPase<sup>old\u201035<\/sup><\/i>)"],"prefix":"10.1002","volume":"229","author":[{"given":"Upneet K.","family":"Sokhi","sequence":"first","affiliation":[{"name":"Department of Human and Molecular Genetics Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manny D.","family":"Bacolod","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Institute of Molecular Medicine Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luni","family":"Emdad","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Institute of Molecular Medicine Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Massey Cancer Center Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swadesh K.","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Institute of Molecular Medicine Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine I.","family":"Dumur","sequence":"additional","affiliation":[{"name":"Department of Pathology Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael F.","family":"Miles","sequence":"additional","affiliation":[{"name":"VCU Massey Cancer Center Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"Department of Pharmacology and Toxicology Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"Department of Neurology Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devanand","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Institute of Molecular Medicine Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Massey Cancer Center Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul B.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Institute of Molecular Medicine Virginia Commonwealth University, School of Medicine Richmond Virginia"},{"name":"VCU Massey Cancer Center Virginia Commonwealth University, School of Medicine Richmond Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,8,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)00805-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029441"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01002-06"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02652"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914143107"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.572"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38609-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20005"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-08-0636"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-5-17"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90499-G"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2010.04.003"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.10.044"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-9-r183"},{"key":"e_1_2_6_17_1","first-page":"256","article-title":"2008. RNA recognition by 3\u2018\u2010to\u20105' exonucleases: The substrate perspective","volume":"4","author":"Ibrahim H","year":"1779","journal-title":"Biochim Et Biophys Acta"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252643699"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2003.07.012"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02035"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr3181"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.092"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1118"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.449"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500804200"},{"key":"e_1_2_6_26_1","article-title":"2007. VENNY","author":"Oliveros JC","year":"2007","journal-title":"An interactive tool for comparing lists with Venn Diagrams."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-011-0576-7"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.2144\/03342mt01"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302421200"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1772"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.16.7333-7343.2005"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2006.04.003"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.10.2741"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.04.009"},{"key":"e_1_2_6_35_1","first-page":"21","article-title":"Epigenetics\u2010related genes in prostate cancer: expression profile in prostate cancer tissues, androgen\u2010sensitive and \u2010insensitive cell lines","volume":"31","author":"Shaikhibrahim Z","year":"2013","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.697308"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407190-2.00005-8"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076284"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp903"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-S3-S24"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042086"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.009605"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.06.035"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2011.10.001"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.24645","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.24645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T13:10:15Z","timestamp":1696597815000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.24645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,27]]},"references-count":43,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1002\/jcp.24645"],"URL":"https:\/\/doi.org\/10.1002\/jcp.24645","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,27]]}}}