{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:49:53Z","timestamp":1790221793384,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T00:00:00Z","timestamp":1416528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM)","award":["U1059"],"award-info":[{"award-number":["U1059"]}]},{"name":"Rh\u00f4ne Alpes Region"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Osteopontin (OPN) and bone sialoprotein (BSP) are coexpressed in osteoblasts and osteoclasts, and display overlapping properties. We used daily injection of parathyroid hormone 1\u201384 (iPTH) over the calvaria of BSP knockout (\u2212\/\u2212) mice to investigate further their functional specificity and redundancy. iPTH stimulated bone formation in both +\/+ and \u2212\/\u2212 mice, increasing to the same degree periosteum, osteoid and total bone thickness. Expression of OPN, osterix, osteocalcin (OCN) and DMP1 was also increased by iPTH in both genotypes. In contrast to +\/+, calvaria cell cultures from \u2212\/\u2212 mice revealed few osteoblast colonies, no mineralization and little expression of OCN, MEPE or DMP1. In contrast, OPN levels were 5\u00d7 higher in \u2212\/\u2212 versus +\/+ cultures. iPTH increased alkaline phosphatase (ALP) activity in cell cultures of both genotypes, with higher OCN and the induction of mineralization in \u2212\/\u2212 cultures. siRNA blocking of OPN expression did not alter the anabolic action of the hormone in BSP\u2009+\/+ calvaria, while it blunted iPTH effects in \u2212\/\u2212 mice, reduced to a modest increase in periosteum thickness. In \u2212\/\u2212 (not +\/+) cell cultures, siOPN blocked the stimulation by iPTH of ALP activity and OCN expression, as well as the induction of mineralization. Thus, full expression of either OPN or BSP is necessary for the anabolic effect of PTH at least in the ectopic calvaria injection model. This suggests that OPN may compensate for the lack of BSP in the response to this hormonal challenge, and provides evidence of functional overlap between these cognate proteins. J. Cell. Physiol. 230: 568\u2013577, 2015. \u00a9 2014 Wiley Periodicals, Inc., A Wiley Company<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24772","type":"journal-article","created":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T10:56:58Z","timestamp":1409137018000},"page":"568-577","source":"Crossref","is-referenced-by-count":31,"title":["Blocking the Expression of Both Bone Sialoprotein (BSP) and Osteopontin (OPN) Impairs the Anabolic Action of PTH in Mouse Calvaria Bone"],"prefix":"10.1002","volume":"230","author":[{"given":"Wafa","family":"Bouleftour","sequence":"first","affiliation":[{"name":"INSERM U1059\/LBTO Universit\u00e9 de Lyon \u2010 Universit\u00e9 Jean Monnet Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guenaelle","family":"Bouet","sequence":"additional","affiliation":[{"name":"INSERM U1059\/LBTO Universit\u00e9 de Lyon \u2010 Universit\u00e9 Jean Monnet Saint\u2010Etienne 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