{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:37:21Z","timestamp":1791261441673,"version":"4.1.0"},"reference-count":94,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T00:00:00Z","timestamp":1435017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Educational Commission of Guangdong","award":["No. 2013CXZDA008"],"award-info":[{"award-number":["No. 2013CXZDA008"]}]},{"name":"the Funds for Key Natural Science Foundation of Guangdong","award":["No.S2013020012754"],"award-info":[{"award-number":["No.S2013020012754"]}]},{"name":"the Natural Science Fund of China","award":["No. 81171179"],"award-info":[{"award-number":["No. 81171179"]}]},{"name":"the Natural Science Fund of China","award":["No. 81272439"],"award-info":[{"award-number":["No. 81272439"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:label\/><jats:p>Astrocytes are critical for ischemic stroke, and understanding their role in mesenchymal stem cell (MSC)\u2010mediated protection against ischemic injury is important. The paracrine capacity of MSCs has been proposed as the principal mechanism contributing to the protection and repair of brain tissue. In the present study, an in vitro oxygen\u2013glucose deprivation (OGD) model was used to mimic ischemic injury. OGD\u2010induced astrocytes were reperfused with MSC\u2010conditioned medium (MSC\u2010CM) or co\u2010cultured with MSCs for 24\u2009h to create an environment abundant in paracrine factors. The results indicated that both situations could protect astrocytes from apoptosis, increase cell metabolic activity, and reduce glial fibrillary acidic protein (GFAP) overexpression; however, the effects of co\u2010culturing with MSCs were more positive. Paracrine factors suppressed the activation of p38 MAPK, JNK, and their downstream targets p53 and STAT1. Inhibition of p38 MAPK, JNK, p53, and STAT1 attenuated astrocyte injury and\/or GFAP upregulation. Activation of p38 MAPK and JNK suppressed the beneficial effects of paracrine factors, resulting in decreased survival and GFAP overexpression. These results suggest that paracrine factors inhibit p38 MAPK and JNK, and most likely by regulating their downstream targets, p53 and STAT1, to promote astrocyte survival associated with GFAP downregulation after ischemic stroke in vitro. J. Cell. Physiol. 230: 2461\u20132475, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24981","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T12:17:30Z","timestamp":1425557850000},"page":"2461-2475","source":"Crossref","is-referenced-by-count":68,"title":["Paracrine Factors Secreted by MSCs Promote Astrocyte Survival Associated With GFAP Downregulation After Ischemic Stroke via p38 MAPK and JNK"],"prefix":"10.1002","volume":"230","author":[{"given":"Weiyi","family":"Huang","sequence":"first","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingke","family":"Lv","sequence":"additional","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijun","family":"Zeng","sequence":"additional","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Anatomy Key Laboratory of Construction and Detection of Guangdong Province Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanfan","family":"Chen","sequence":"additional","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Key Laboratory of Construction and Detection of Guangdong Province Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Key Laboratory of Construction and Detection of Guangdong Province Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Anatomy Key Laboratory of Construction and Detection of Guangdong Province Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Jiang","sequence":"additional","affiliation":[{"name":"The National Key Clinic Specialty The Neurosurgery Institute of Guangdong Province Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.187"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2010.08.023"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2013.109"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6273-11.2012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0449-07.2007"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2007.04.028"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00211"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI69244"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2009.04.015"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2013.03.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.106.031617"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00220.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.180"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00272"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24212"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-013-1110-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06691.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2014.06.009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2011.0488"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1560"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802091"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.06.091"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.10.069"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22577"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.176826"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23993"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.03.005"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.534388"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12466"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt443"},{"key":"e_1_2_7_33_1","first-page":"3829","article-title":"STAT\u20101 expression in human glioblastoma and peritumoral tissue","volume":"27","author":"Haybaeck J","year":"2007","journal-title":"Anticancer Res"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1440"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2012.02.003"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2014.07.020"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/STR.0b013e318284056a"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22566"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-014-0167-6"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.06.011"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2006.09.003"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00534-009-0155-x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601913507951"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nurt.2010.07.006"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.09.020"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.6.9"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12202"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.09.062"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368913X667709"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1159\/000331739"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-05-432674"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-135"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-013-9920-0"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2009.10.002"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.169"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.110.598763"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04769.x"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2012.2430"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-010-9171-0"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.06.123"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0444"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.2.320-344.2004"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-396456-4.00013-4"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-011-0040-6"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.12.6586"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065446"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2012.04.018"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-012-9798-2"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00536.2007"},{"key":"e_1_2_7_70_1","first-page":"1604","article-title":"Resveratrol\u2010induced activation of p53 and apoptosis is mediated by extracellular\u2010signal\u2010regulated protein kinases and p38 kinase","volume":"61","author":"She QB","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1752-8062.2011.00283.x"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.20.13985"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-195719"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2009.08.002"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-8-26"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.11.019"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072926"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1808"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0465"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00171-9"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2694"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.02.068"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.12.004"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037574"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.02.046"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.116"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-014-0320-9"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2009.08.096"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07446.x"},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X550198"},{"key":"e_1_2_7_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.02.047"},{"key":"e_1_2_7_92_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6221-11.2012"},{"key":"e_1_2_7_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nurt.2010.07.004"},{"key":"e_1_2_7_94_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04957.x"},{"key":"e_1_2_7_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2014.03.030"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.24981","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.24981","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T21:16:28Z","timestamp":1696367788000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.24981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,23]]},"references-count":94,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1002\/jcp.24981"],"URL":"https:\/\/doi.org\/10.1002\/jcp.24981","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,23]]}}}