{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T03:33:35Z","timestamp":1768448015259,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T00:00:00Z","timestamp":1435017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81160046"],"award-info":[{"award-number":["81160046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guangxi Scientific Research and Technological Development Program","award":["1140003A-19"],"award-info":[{"award-number":["1140003A-19"]}]},{"name":"Innovation Project of Guangxi Graduate Education","award":["2010105981002M174"],"award-info":[{"award-number":["2010105981002M174"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:label\/><jats:p>An imbalance between T helper 1 (Th1) and T helper 2 (Th2) cells has been reported to increase plaque instability in patients with unstable angina (UA). MicroRNAs play a vital role in the differentiation of CD4<jats:sup>+<\/jats:sup> T cells. However, the role of microRNAs in regulation of Th1\/Th2 balance in UA remains unclear. In this study, we aimed to elucidate microRNA expression profiles of circulating CD4<jats:sup>+<\/jats:sup> T cells in UA and to explore the function of microRNAs in the Th1\/Th2 balance. A total of 53 patients with UA and 31 control subjects without coronary artery disease were enrolled. Microarray analysis of the microRNA expression profiles of CD4<jats:sup>+<\/jats:sup> T cells revealed that <jats:italic>miR\u2010155<\/jats:italic> was the most significantly upregulated microRNA of the 451 differentially expressed microRNAs. The upregulation of <jats:italic>miR\u2010155<\/jats:italic> expression was positively correlated with the percentage of Th1 cells and interferon\u2010gamma (IFN\u2010\u03b3) levels in patients with UA. In addition, overexpression of <jats:italic>miR\u2010155<\/jats:italic> in human circulating CD4<jats:sup>+<\/jats:sup> T cells promoted Th1 differentiation. Further studies identified IFN\u2010\u03b3 receptor alpha chain (<jats:italic>IFN\u2010\u03b3 R\u03b1<\/jats:italic>) mRNA as a direct and functional target of <jats:italic>miR\u2010155<\/jats:italic>. A luciferase reporter assay verified that <jats:italic>miR\u2010155<\/jats:italic> directly targeted <jats:italic>IFN\u2010\u03b3 R\u03b1<\/jats:italic> mRNA. Small\u2010interfering RNA\u2010mediated knockdown of <jats:italic>IFN\u2010\u03b3 R\u03b1<\/jats:italic> mRNA showed effects similar to those of ectopic <jats:italic>miR\u2010155<\/jats:italic> expression. Thus, our study indicated that upregulation of <jats:italic>miR\u2010155<\/jats:italic> in circulating CD4<jats:sup>+<\/jats:sup> T cells in patients with UA promoted a shift in the Th1\/Th2 balance toward Th1 dominance by repressing IFN\u2010\u03b3 R\u03b1, which may subsequently enhance plaque instability. J. Cell. Physiol. 230: 2498\u20132509, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.24987","type":"journal-article","created":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T11:14:49Z","timestamp":1426072489000},"page":"2498-2509","source":"Crossref","is-referenced-by-count":19,"title":["Upregulation of <i>miR\u2010155<\/i> in CD4<sup>+<\/sup> T Cells Promoted Th1 Bias in Patients With Unstable Angina"],"prefix":"10.1002","volume":"230","author":[{"given":"Yanmei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Guangxi Medical University Nanning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology The First Affiliated Hospital of Guangxi Medical University Nanning 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