{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T16:16:49Z","timestamp":1771517809478,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T00:00:00Z","timestamp":1448236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACyT-Mexico)","award":["128566"],"award-info":[{"award-number":["128566"]}]},{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda (CONACyT-Mexico)","award":["84362"],"award-info":[{"award-number":["84362"]}]},{"name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico\/Universidad Nacional Aut\u00f3noma de M\u00e9xico (DGAPA\/UNAM)","award":["IN204914"],"award-info":[{"award-number":["IN204914"]}]},{"name":"The Alexander von Humboldt Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:sec><jats:label\/><jats:p>Zn<jats:sup>2+<\/jats:sup> is a trace metal which is important for spermatogenesis progression; its deficiency causes atrophy or malignant growth of the testis. Although testis, epididymis, and prostate contain high Zn<jats:sup>2+<\/jats:sup> concentrations, the molecular entities which are modulated by this metal are still under study. Interestingly, spermatogenic cells mainly express Ca<jats:sub>V<\/jats:sub>3.2\u2010encoded T\u2010type Ca<jats:sup>2+<\/jats:sup> currents (I<jats:sub>CaT<\/jats:sub>) which are positively or negatively modulated by Zn<jats:sup>2+<\/jats:sup> in other tissues. To explore whether I<jats:sub>CaT<\/jats:sub> could be regulated by Zn<jats:sup>2+<\/jats:sup> and albumin, its main physiological carrier, we performed whole cell electrophysiological recordings of spermatogenic cell I<jats:sub>CaT<\/jats:sub> in the absence or presence of different Zn<jats:sup>2+<\/jats:sup> concentrations. Zn<jats:sup>2+<\/jats:sup> decreased I<jats:sub>CaT<\/jats:sub> in a concentration\u2010dependent manner (IC<jats:sub>50<\/jats:sub>\u2009= 2\u2009\u03bcM) and this inhibition could only be completely removed in presence of albumin. Differently to previous reports, I<jats:sub>CaT<\/jats:sub> did not show a tonic inhibition by Zn<jats:sup>2+<\/jats:sup>. Further analysis showed that Zn<jats:sup>2+<\/jats:sup> did not affect the voltage dependency or the kinetics of current activation, but right shifted the steady\u2010state inactivation curve and slowed inactivation and deactivation kinetics. Recovery from inactivation was also altered. However, these apparent alterations in gating properties are not enough to explain the strong I<jats:sub>CaT<\/jats:sub> reduction. Using non\u2010stationary fluctuation analysis, we found that Zn<jats:sup>2+<\/jats:sup> mainly reduced the number of available Ca<jats:sup>2+<\/jats:sup> channels without changing the single channel current amplitude. I<jats:sub>CaT<\/jats:sub> modulation by Zn<jats:sup>2+<\/jats:sup> could be relevant for spontaneous Ca<jats:sup>2+<\/jats:sup> oscillations during spermatogenesis and in pathophysiological conditions such as diabetes. J. Cell. Physiol. 231: 659\u2013667, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25112","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T14:48:03Z","timestamp":1438181283000},"page":"659-667","source":"Crossref","is-referenced-by-count":7,"title":["Regulation of Spermatogenic Cell T\u2010Type Ca<sup>2+<\/sup> Currents by Zn<sup>2+<\/sup>: Implications in Male Reproductive Physiology"],"prefix":"10.1002","volume":"231","author":[{"given":"Ignacio","family":"L\u00f3pez\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular Instituto de Biotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Cuernavaca Morelos M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia L.","family":"Trevi\u00f1o","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y 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