{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:28:37Z","timestamp":1791246517057,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,9,29]],"date-time":"2015-09-29T00:00:00Z","timestamp":1443484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["2013\/19985-7"],"award-info":[{"award-number":["2013\/19985-7"]}]},{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["2013\/22737-5"],"award-info":[{"award-number":["2013\/22737-5"]}]},{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["2013\/20591-3"],"award-info":[{"award-number":["2013\/20591-3"]}]},{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["2014\/25459-9"],"award-info":[{"award-number":["2014\/25459-9"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>The aim of this study was to verify the effects of running overtraining protocols performed in downhill, uphill, and without inclination on the proteins related to hypertrophy signaling pathway in extensor digitorum longus (EDL) and soleus of C57BL\/6 mice. We also performed histological and stereological analyses. Rodents were divided into control (CT; sedentary mice), overtrained by downhill running (OTR\/down), overtrained by uphill running (OTR\/up), and overtrained by running without inclination (OTR). The incremental load, exhaustive, and grip force tests were used as performance evaluation parameters. 36\u2009h after the grip force test, EDL and soleus were removed and immediately used for immunoblotting analysis or stored at \u221280\u00b0C for histological and stereological analyses. For EDL, OTR\/down decreased the protein kinase B (Akt) and tuberous sclerosis protein 2 (TSC2) phosphorylation (p), and increased myostatin, receptor\u2010activated Smads (pSMAD2\u20103), and insulin receptor substrate\u20101 (pIRS\u20101; Ser307\/636). OTR\/down also presented low and high relative proportions of cytoplasm and connective tissue, respectively. OTR\/up increased the mammalian target of rapamycin (pmTOR), 70\u2010kDa ribosomal protein S6 kinase 1 (pS6K1) and pSMAD2\u20103, and decreased pTSC2. OTR decreased pTSC2 and increased pIRS\u20101 (Ser636). For soleus, OTR\/down increased S6 ribosomal protein (pS6RP) and pSMAD2\u20103, and decreased pIRS\u20101 (Ser639). OTR\/up decreased pS6K1, pS6RP and pIRS\u20101 (Ser639), and increased pTSC2 (Ser939), and pSMAD2\u20103. OTR increased pS6RP, 4E\u2010binding protein\u20101 (p4E\u2010BP1), pTSC2 (Ser939), and pSMAD2\u20103, and decreased pIRS\u20101 (Ser639). In summary, OTR\/down inhibited the skeletal muscle hypertrophy with concomitant signs of atrophy in EDL. The effects of OTR\/up and OTR depended on the analyzed skeletal muscle type. J. Cell. Physiol. 231: 1045\u20131056, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25197","type":"journal-article","created":{"date-parts":[[2015,9,18]],"date-time":"2015-09-18T08:07:38Z","timestamp":1442563658000},"page":"1045-1056","source":"Crossref","is-referenced-by-count":50,"title":["Downhill Running Excessive Training Inhibits Hypertrophy in Mice Skeletal Muscles with Different Fiber Type Composition"],"prefix":"10.1002","volume":"231","author":[{"given":"Alisson L.","family":"da Rocha","sequence":"first","affiliation":[{"name":"Postgraduate Program in Rehabilitation and Functional Performance Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo (USP) Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno C.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Rehabilitation and Functional Performance Ribeir\u00e3o Preto Medical School 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