{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:08:55Z","timestamp":1792782535878,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,3,16]],"date-time":"2016-03-16T00:00:00Z","timestamp":1458086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Suzuken Memorial Foundation Grant-in-aid [J.L.]","id":[{"id":"https:\/\/ror.org\/04vs2x012","id-type":"ROR","asserted-by":"crossref"}]},{"name":"TERUMO Research Fellowship [J.L.]"},{"name":"Magnetic Health Science Foundation [K.M.]","id":[{"id":"https:\/\/ror.org\/03s8dv541","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Skeletal myoblast (SkMB) transplantation has been conducted as a therapeutic strategy for severe heart failure. However, arrhythmogenicity following transplantation remains unsolved. We developed an in vitro model of myoblast transplantation with \u201cpatterned\u201d or \u201crandomly\u2010mixed\u201d co\u2010culture of SkMBs and cardiomyocytes enabling subsequent electrophysiological, and arrhythmogenic evaluation. SkMBs were magnetically labeled with magnetite nanoparticles and co\u2010cultured with neonatal rat ventricular myocytes (NRVMs) on multi\u2010electrode arrays. SkMBs were patterned by a magnet beneath the arrays. Excitation synchronicity was evaluated by Ca\n                      <jats:sup>2+<\/jats:sup>\n                      imaging using a gene\u2010encoded Ca\n                      <jats:sup>2+<\/jats:sup>\n                      indicator, G\u2010CaMP2. In the monoculture of NRVMs (control), conduction was well\u2010organized. In the randomly\u2010mixed co\u2010culture of NRVMs and SkMBs (random group), there was inhomogeneous conduction from multiple origins. In the \u201cpatterned\u201d co\u2010culture where an en bloc SKMB\u2010layer was inserted into the NRVM\u2010layer, excitation homogenously propagated although conduction was distorted by the SkMB\u2010area. The 4\u2010mm distance conduction time (CT) in the random group was significantly longer (197\u2009\u00b1\u2009126\u2009ms) than in control (17\u2009\u00b1\u20093\u2009ms). In the patterned group, CT through NRVM\u2010area did not change (25\u2009\u00b1\u20093\u2009ms), although CT through the SkMB\u2010area was significantly longer (132\u2009\u00b1\u200977\u2009ms). The intervals between spontaneous excitation varied beat\u2010to\u2010beat in the random group, while regular beating was recorded in the control and patterned groups. Synchronized Ca\n                      <jats:sup>2+<\/jats:sup>\n                      transients of NRVMs were observed in the patterned group, whereas those in the random group were asynchronous. Patterned alignment of SkMBs is feasible with magnetic nanoparticles. Using the novel in vitro model mimicking cell transplantation, it may become possible to predict arrhythmogenicity due to heterogenous cell transplantation. J. Cell. Physiol. 231: 2249\u20132256, 2016. \u00a9 2016 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.25342","type":"journal-article","created":{"date-parts":[[2016,2,13]],"date-time":"2016-02-13T07:31:35Z","timestamp":1455348695000},"page":"2249-2256","source":"Crossref","is-referenced-by-count":2,"title":["A New In Vitro Co\u2010Culture Model Using Magnetic Force\u2010Based Nanotechnology"],"prefix":"10.1002","volume":"231","author":[{"given":"Hiroki","family":"Takanari","sequence":"first","affiliation":[{"name":"Department of Pathophysiology Oita University School of Medicine Oita Japan"},{"name":"Department of Cardiovascular Research Research Institute of Environmental Medicine Nagoya University Nagoya Japan"},{"name":"Division of Heart and Lungs Department of Medical Physiology University Medical Center Utrecht Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Miwa","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Research Research Institute of Environmental Medicine Nagoya University Nagoya Japan"},{"name":"Department of Cardiovascular Regenerative Medicine Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XianMing","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Research Research Institute of Environmental Medicine Nagoya University Nagoya Japan"},{"name":"Department of Cardiothoracic Surgery Graduate School of Medicine Nagoya University Nagoya Japan"},{"name":"Department of Cardiovascular Surgery Central South University The Second Xiangya Hospital Changsha 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Tonomura","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Ritsumeikan University Kusatsu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Konishi","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Ritsumeikan University Kusatsu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Opthof","sequence":"additional","affiliation":[{"name":"Division of Heart and Lungs Department of Medical Physiology University Medical Center Utrecht Utrecht The Netherlands"},{"name":"Experimental Cardiology Group Center for Heart Failure Research Academic Medical Center Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel AG","family":"van der Heyden","sequence":"additional","affiliation":[{"name":"Division of Heart and Lungs Department of Medical Physiology University Medical Center Utrecht Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itsuo","family":"Kodama","sequence":"additional","affiliation":[{"name":"Nagoya University Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong\u2010Kook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Regenerative Medicine Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000174794.22491.a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.547810"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4244\/EIJV6I7A139"},{"key":"e_1_2_8_5_1","first-page":"108","article-title":"skeletal myoblast 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