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In the present study, we investigated whether ginkgolic acid can affect EMT in lung cancer cells and the related underlying mechanism(s) of its actions. We found that ginkgolic acid C15:1 (GA C15:1) inhibited cell proliferation, invasion, and migration in both A549 and H1299 lung cancer cells. GA C15:1 also suppressed the expression of EMT related genes (Fibronectin, Vimentin, N\u2010cadherin, MMP\u20109, MMP\u20102, Twist and Snail) and suppressed TGF\u2010\u03b2\u2010induced EMT as assessed by reduced expression of mesenchymal markers (Fibronectin, Vimentin, N\u2010cadherin), MMP\u20109, MMP\u20102, Twist and Snail. However, GA C15:1 did not affect the expression of various epithelial marker proteins (Occludin and E\u2010cadherin) in both A549 and H1299 cells. TGF\u2010\u03b2\u2010induced morphologic changes from epithelial to mesenchymal cells and induction of invasion and migration were reversed by GA C15:1. Finally, GA C15:1 not only abrogated basal PI3K\/Akt\/mTOR signaling cascade, but also reduced TGF\u2010\u03b2\u2010induced phosphorylation of PI3K\/Akt\/mTOR pathway in lung cancer cells. Overall, these findings suggest that GA C15:1 suppresses lung cancer invasion and migration through the inhibition of PI3K\/Akt\/mTOR signaling pathway and provide a source of potential therapeutic compounds to control the metastatic dissemination of tumor cells. J. Cell. Physiol. 232: 346\u2013354, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25426","type":"journal-article","created":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T20:56:44Z","timestamp":1463173004000},"page":"346-354","source":"Crossref","is-referenced-by-count":183,"title":["Ginkgolic Acid Inhibits Invasion and Migration and TGF\u2010\u03b2\u2010Induced EMT of Lung Cancer Cells Through PI3K\/Akt\/mTOR Inactivation"],"prefix":"10.1002","volume":"232","author":[{"given":"Seung Ho","family":"Baek","sequence":"first","affiliation":[{"name":"College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong\u2010Hyeon","family":"Ko","sequence":"additional","affiliation":[{"name":"College of Korean Medicine Kyung Hee University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong 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