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Abnormal distribution\u2010changes and dysfunctions in such tissue are associated to different pathologies. Adipocytes are generated from progenitor cells, via a complex differentiating process not yet well understood. Therefore, we investigated differential mRNA and miRNA expression patterns of human mesenchymal stromal\u2010cells (MSC) induced and not induced to differentiate into adipocytes by next (second)\u2010generation sequencing. A total of 2,866 differentially expressed genes (101 encoding miRNA) were identified, with 705 (46 encoding miRNA) being upregulated in adipogenesis. They were related to different pathways, including PPARG, lipid, carbohydrate and energy metabolism, redox, membrane\u2010organelle biosynthesis, and endocrine system. Downregulated genes were related to extracellular matrix and cell migration, proliferation, and differentiation. Analyses of mRNA\u2010miRNA interaction showed that repressed miRNA\u2010encoding genes can act downregulating PPARG\u2010related genes; mostly the PPARG activator (\n                      <jats:italic>PPARGC1A<\/jats:italic>\n                      ). Induced miRNA\u2010encoding genes regulate downregulated genes related to\n                      <jats:italic>TGFB1<\/jats:italic>\n                      . These results shed new light to understand adipose\u2010tissue differentiation and physiology, increasing our knowledge about pathologies like obesity, type\u20102 diabetes and osteoporosis. J. Cell. 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