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Expression of the enhanced green fluorescent protein (EGFP) driven by the second intron of the nestin gene identified a subpopulation of EGFP\/nestin<jats:sup>(+)<\/jats:sup> cells that differentiated to a vascular phenotype in the peri\u2010infarct\/infarct region of post\u2010MI mice albeit the transgene was not detected in nestin<jats:sup>(+)<\/jats:sup>\u2010cardiomyocytes. \u03b1\u2010MHC\u2010driven expression of the reporter mCherry was detected in troponin\u2010T<jats:sup>(+)<\/jats:sup>\u2010 and nestin<jats:sup>(+)<\/jats:sup>\u2010cardiomyocytes in the peri\u2010infarct\/infarct region of post\u2010MI mice. However, the cell cycle re\u2010entry of nestin\/mCherry<jats:sup>(+)<\/jats:sup>\u2010cardiomyocytes was not observed. Nestin staining was identified in a paucity of neonatal rat ventricular cardiomyocytes (NNVM). Exposure to phorbol 12,13\u2010dibutyrate (PDBu) induced NNVM hypertrophy but did not promote nestin expression or Brdu incorporation. PDBu treatment of NNVMs phosphorylated p38 MAPK and HSP27 and HSP27 phosphorylation was abrogated by the p38 MAPK inhibitor SB203580. PDBu\/SB203580 co\u2010treatment significantly increased the percentage of NNVMs that expressed nestin and incorporated Brdu. In the heart of embryonic 10.5\u2009day mice, nestin immunoreactivity was observed in cycling troponin\u2010T<jats:sup>(+)<\/jats:sup>\u2010cardiomyocytes. Nestin was also detected in embryonic rat ventricular cardiomyocytes and depletion of the intermediate filament protein attenuated cell cycle re\u2010entry. Thus, nestin expressed by pre\u2010existing cardiomyocytes following ischemic damage recapitulated in part an embryonic trait and may provide the requisite phenotype to initiate cell cycle re\u2010entry. However, the overt activation of the p38 MAPK pathway post\u2010MI may in part limit the appearance and inhibit the cell cycle re\u2010entry of nestin<jats:sup>(+)<\/jats:sup>\u2010cardiomyocytes. J. Cell. Physiol. 232: 1717\u20131727, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25496","type":"journal-article","created":{"date-parts":[[2016,7,20]],"date-time":"2016-07-20T18:26:42Z","timestamp":1469039202000},"page":"1717-1727","source":"Crossref","is-referenced-by-count":21,"title":["Nestin Expressed by Pre\u2010Existing Cardiomyocytes Recapitulated in Part an Embryonic Phenotype; Suppressive Role of p38 MAPK"],"prefix":"10.1002","volume":"232","author":[{"given":"Marc\u2010Andre","family":"Meus","sequence":"first","affiliation":[{"name":"Montreal Heart Institute Universit\u00e9 de Montr\u00e9al Montr\u00e9al Qu\u00e9bec Canada"},{"name":"D\u00e9partement de Physiologie Mol\u00e9culaire et Int\u00e9grative Universit\u00e9 de Montr\u00e9al Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Hertig","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute 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