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Here we describe how apoptotic cells can use intercellular membrane nanotubes to transfer exposed PS to neighboring viable cells, and thus deposit an \u201ceat\u2010me\u201d tag on the viable cells. Tunneling nanotubes (TNTs) connected UV\u2010treated apoptotic rat pheochromocytoma PC12 cells with neighboring untreated cells. These TNTs were composed of PS\u2010exposed plasma membrane and facilitated the transfer of the membrane from apoptotic to viable cells. Other pro\u2010phagocytic signals, such as oxidized phospholipids and calreticulin, were also transferred to viable cells. In addition, anti\u2010phagocytic signal CD47 presenting on the plasma membrane of viable cells was masked by the transferred PS\u2010membrane. Confocal imaging revealed an increase of phagocytosis of viable PC12 cells by murine RAW264.7 macrophages when the viable PC12 cells were cocultured with UV\u2010treated PC12 cells. Treatment with 50\u2009nM cytochalasin D would abolish TNTs and correspondingly inhibit this phagocytosis of the viable cells. Our study indicates that exposed\u2010PS membrane is delivered from apoptotic to viable cells through TNTs. This transferred membrane may act as a pro\u2010phagocytic signal for macrophages to induce phagocytosis of viable cells in a situation where they are in the vicinity of apoptotic cells. J. Cell. Physiol. 232: 2271\u20132279, 2017. \u00a9 2016 The Authors. <jats:italic>Journal of Cellular Physiology<\/jats:italic> Published by Wiley Periodicals Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25584","type":"journal-article","created":{"date-parts":[[2016,9,3]],"date-time":"2016-09-03T21:02:42Z","timestamp":1472936562000},"page":"2271-2279","source":"Crossref","is-referenced-by-count":35,"title":["TNT\u2010Induced Phagocytosis: Tunneling Nanotubes Mediate the Transfer of Pro\u2010Phagocytic Signals From Apoptotic to Viable Cells"],"prefix":"10.1002","volume":"232","author":[{"given":"Margarethe","family":"Bittins","sequence":"first","affiliation":[{"name":"Department of Biomedicine University of Bergen Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedicine University of Bergen Bergen 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