{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:20:41Z","timestamp":1792707641732,"version":"4.5.0"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,4,10]],"date-time":"2017-04-10T00:00:00Z","timestamp":1491782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 GM 30758"],"award-info":[{"award-number":["RO1 GM 30758"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:label\/><jats:p>In live cell imaging of fluorescent proteins, phototoxicity of the excitation light can be problematical. Cell death is obvious, but reduced cell viability can make the interpretation of observations error prone. We characterized the phototoxic consequences of 488 and 546\u2009nm light on untransformed human cells and tested methods that have or could be used to alleviate photodamage. Unlabeled RPE1 cells were given single 0.5\u20132.5\u2009min irradiations in early G1 from a mercury arc lamp on a fluorescence microscope. Four hundred eighty\u2010eight nanometer light produced a dose\u2010dependent decrease in the percentage of cells that progressed to mitosis, slowing of the cell cycle for some of those entering mitosis, and a \u223c12% incidence of cell death for the highest dose. For 546\u2009nm light we found a 10\u201315% reduction in the percentage of cells entering mitosis, no strong dose dependency, and a \u223c2% incidence of cell death for the longest irradiations. For cells expressing GFP\u2010centrin1 or mCherry\u2010centrin1, fewer entered mitosis for each dose than unlabeled cells. For constant total dose 488\u2009nm light irradiations of unlabeled cells, reducing the intensity 10\u2010fold or spreading the exposures out as a series of 10\u2009sec pulses at 1\u2009min intervals produced a minor and not consistent improvement in the percentage of cells entering mitosis. Reducing oxidative processes, by culturing at \u223c3% oxygen or adding the reducing agent Trolox noticeably increased the fraction of cells entering mitosis. Thus, for long\u2010term imaging there can be value to using RFP constructs and for GFP\u2010tagged proteins reducing oxidative processes. J. Cell. Physiol. 232: 2461\u20132468, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25588","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T12:47:07Z","timestamp":1473338827000},"page":"2461-2468","source":"Crossref","is-referenced-by-count":64,"title":["Live Cell Imaging: Assessing the Phototoxicity of 488 and 546\u2009nm Light and Methods to Alleviate it"],"prefix":"10.1002","volume":"232","author":[{"given":"Stephen","family":"Douthwright","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greenfield","family":"Sluder","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407761-4.00019-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004037107"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01258.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407761-4.00020-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01868.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.894001"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1278"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.3.585"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.1.237"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05223"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407761-4.00023-3"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407761-4.00016-6"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1057330"},{"key":"e_1_2_6_15_1","first-page":"345","volume-title":"Live cell imaging: A laboratory manual","author":"Sluder G","year":"2004"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1082160"},{"key":"e_1_2_6_17_1","first-page":"329","volume-title":"Live cell imaging: A laboratory manual","author":"Swedlow JR","year":"2005"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-391856-7.00039-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200403014"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.08.018"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200607073"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407761-4.00006-3"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1993.tb00091.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1990.tb01685.x"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.25588","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.25588","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.25588","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T10:51:36Z","timestamp":1696330296000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.25588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,10]]},"references-count":23,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/jcp.25588"],"URL":"https:\/\/doi.org\/10.1002\/jcp.25588","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,10]]}}}