{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:53Z","timestamp":1786978013539,"version":"build-2736575974"},"reference-count":83,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,12,29]],"date-time":"2016-12-29T00:00:00Z","timestamp":1482969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP\u2010740"],"award-info":[{"award-number":["PIP\u2010740"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec>\n                    <jats:label\/>\n                    <jats:p>Epithelial Cadherin (E\u2010cadherin) is involved in calcium\u2010dependent cell\u2013cell adhesion and signal transduction. The E\u2010cadherin decrease\/loss is a hallmark of Epithelial to Mesenchymal Transition (EMT), a key event in tumor progression. The underlying molecular mechanisms that trigger E\u2010cadherin loss and consequent EMT have not been completely elucidated. This study reports the identification of a novel human E\u2010cadherin variant mRNA produced by alternative splicing. A bioinformatics evaluation of the novel mRNA sequence and biochemical verifications suggest its regulation by Nonsense\u2010Mediated mRNA Decay (NMD). The novel E\u2010cadherin variant was detected in 29\/42 (69%) human tumor cell lines, expressed at variable levels (E\u2010cadherin variant expression relative to the wild type mRNA\u2009=\u20090.05\u201311.6%). Stable transfection of the novel E\u2010cadherin variant in MCF\u20107 cells (MCF7Ecadvar) resulted in downregulation of wild type E\u2010cadherin expression (transcript\/protein) and EMT\u2010related changes, among them acquisition of a fibroblastic\u2010like cell phenotype, increased expression of Twist, Snail, Zeb1, and Slug transcriptional repressors and decreased expression of ESRP1 and ESRP2 RNA binding proteins. Moreover, loss of cytokeratins and gain of vimentin, N\u2010cadherin and Dysadherin\/FXYD5 proteins was observed. Dramatic changes in cell behavior were found in MCF7Ecadvar, as judged by the decreased cell\u2013cell adhesion (Hanging\u2010drop assay), increased cell motility (Wound Healing) and increased cell migration (Transwell) and invasion (Transwell w\/Matrigel). Some changes were found in MCF\u20107 cells incubated with culture medium supplemented with conditioned medium from HEK\u2010293 cells transfected with the E\u2010cadherin variant mRNA. Further characterization of the novel E\u2010cadherin variant will help understanding the molecular basis of tumor progression and improve cancer diagnosis. J. Cell. Physiol. 232: 1368\u20131386, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcp.25622","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T16:57:29Z","timestamp":1475081849000},"page":"1368-1386","source":"Crossref","is-referenced-by-count":31,"title":["Identification of a Novel Human E\u2010Cadherin Splice Variant and Assessment of Its Effects Upon EMT\u2010Related Events"],"prefix":"10.1002","volume":"232","author":[{"given":"Mar\u00eda Laura","family":"Matos","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME). National Research Council of Argentina (CONICET). 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