{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T07:17:44Z","timestamp":1720595864310},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,11,10]],"date-time":"2016-11-10T00:00:00Z","timestamp":1478736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Health","award":["EY006915"],"award-info":[{"award-number":["EY006915"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal Cellular Physiology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:label \/><jats:p>Insulin has been shown to elicit changes of Na,K\u2010ATPase activity in various tissues. Na,K\u2010ATPase in the nonpigmented ciliary epithelium (NPE) plays a role in aqueous humor secretion and changes of Na,K\u2010ATPase activity impact the driving force. Because we detect a change of NPE Na,K\u2010ATPase activity in response to insulin, studies were carried out to examine the response mechanism. Ouabain\u2010sensitive rubidium (Rb) uptake by cultured NPE cells, measured as a functional index of Na,K\u2010ATPase\u2010mediated inward potassium transport, was found to increase in cells exposed for 5\u2009min to insulin. The maximally effective concentration was 100\u2009nM. An intrinsic increase of Na,K\u2010ATPase activity evident as a &gt;2\u2010fold increase in the rate of ouabain\u2010sensitive ATP hydrolysis in homogenates obtained from cells exposed to 100\u2009nM insulin for 5\u2009min was also observed. Insulin\u2010treated cells exhibited Akt, Src family kinase (SFK), ERK1\/2, and p38 activation, all of which were prevented by a pI3 kinase inhibitor LY294002. The Rb uptake and Na,K\u2010ATPase activity response to insulin both were abolished by PP2, an SFK inhibitor which also prevented p38 and ERK1\/2 but not Akt activation. The Akt inhibitor MK\u20102206 did not change the Na,K\u2010ATPase response to insulin. The findings suggest insulin activates pI3K\u2010dependent Akt and SFK signaling pathways that are separate. ERK1\/2 and p38 activation is secondary to and dependent on SFK activation. The increase of Na,K\u2010ATPase activity is dependent on activation of the SFK pathway. The findings are consistent with previous studies that indicate a link between Na,K\u2010ATPase activity and SFK signaling. J. Cell. Physiol. 232: 1489\u20131500, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcp.25654","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T11:49:54Z","timestamp":1476704994000},"page":"1489-1500","source":"Crossref","is-referenced-by-count":3,"title":["Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K\u2010ATPase in Nonpigmented Ciliary Epithelium"],"prefix":"10.1002","volume":"232","author":[{"given":"Mohammad","family":"Shahidullah","sequence":"first","affiliation":[{"name":"Department of Physiology and Ophthalmology and Vision Science University of Arizona Tucson Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amritlal","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Physiology and Ophthalmology and Vision Science University of Arizona Tucson Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas A.","family":"Delamere","sequence":"additional","affiliation":[{"name":"Department of Physiology and Ophthalmology and Vision Science University of Arizona Tucson Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402152200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07228.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(97)00231-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2012.07.006"},{"key":"e_1_2_6_6_1","first-page":"43","volume-title":"Vascular complications of diabetes mellitus","author":"Becker B","year":"1967"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1966.03850010479003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.066464"},{"key":"e_1_2_6_9_1","first-page":"1524","article-title":"Insulin concentration in aqueous humor after paracentesis and feeding of rabbits","volume":"19","author":"Coulter JB","year":"1980","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(83)90074-X"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1965.tb05443.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(96)30511-3"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.1016\/S0021-9258(19)39891-6","article-title":"Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K\u2010ATPase in the ocular ciliary epithelium","volume":"265","author":"Ghosh S","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_6_14_1","first-page":"91","article-title":"Intraocular pressure in young diabetics and its relationship with diabetic retinopathy","volume":"17","author":"Jain IS","year":"1969","journal-title":"J All India Ophthalmol Soc"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1967.00980030200013"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311715200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(94)31191-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1529-1839(04)70038-X"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2415-10-19"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(01)01048-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-6503fje"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/09286589609080113"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02456527"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22602"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(97)30247-4"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3109\/03009739709178930"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2720727"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2006.01.066"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-7634(05)80004-1"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.2.C311"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/10799893.2011.587821"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00010.2012"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24454"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/02713680701434899"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00057.2013"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.00312.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006805510749"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00579.2006"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(95)31055-X"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/6.7.626"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90470-3"}],"container-title":["Journal of Cellular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.25654","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcp.25654","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcp.25654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T04:21:33Z","timestamp":1694924493000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcp.25654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,10]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1002\/jcp.25654"],"URL":"https:\/\/doi.org\/10.1002\/jcp.25654","archive":["Portico"],"relation":{},"ISSN":["0021-9541","1097-4652"],"issn-type":[{"value":"0021-9541","type":"print"},{"value":"1097-4652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,10]]}}}